木耳漆酶高产菌株筛选及其发酵条件的研究

Study on Screening for High Yield Laccse Producing Strains from Auricularia and Its Optimum Fermentation conditions

通过运用不同检测方法对木耳属中的三个种的 2 7个菌株的产过氧化物酶、漆酶能力的检测 ,筛选得到一漆酶高产菌株毛木耳 (Auriculariapolytricha)AP4。并且对AP4的产漆酶的发酵条件进行了初步研究。摇瓶实验产漆酶的最佳培养基的成分为 :碳源羧甲基纤维素(CMC) 5g/L ,氮源NH4NO3 +L -天冬酰胺 (L -As paragine) 2 4mmol/L ,培养基的初始pH4 0 ,培养温度2 5℃。

Using different methods to detect superoxidase and laccase activity produced by 27 strians belong to three isolate in Auricularia,we got a high yield producing laccase strain—AP4.Then we studied the fermentation conditions of produing laccase from AP4.The results showed that the composition of optimal fermentation medium was CMC5g/L,NH_4NO_3+L-Asparagine 24mmol/L;Medium pH was 4.0,and culture temperature was 25℃.The experiment supplied the better strain and optimal fermentation conditions of producting laccase for the future study on laccase charactreistics and molecular biology.