牛血Cu/Zn-SOD的热稳定性

Heat Stability of Cu/ Zn-SOD in Cattle Blood

研究了不同的Cu2 + 和Zn2 + 浓度配比对SOD热稳定性的影响 ,并测定了SOD活性及利用考马斯亮蓝G 2 5 0法测定蛋白质含量。结果表明在Cu2 + 、Zn2 + 分别为 5 4mmol L和 3 6mmol L时SOD的活性最高 ,达到了 92 6% ;在 75℃时大部分杂蛋白变性沉淀 ,SOD的比活力最高。最后确定在加入 5 4mmol LCu2 + ,3 6mmol LZn2 + 条件下 ,75℃下热变性 ,可以取得比较高活性和比活性的SOD。从而为寻找更简便、经济的提纯方法提供参考依据。

This study investigated effect of different concentrations of Cu^(2+) and Zn^(2+) on heat stability of SOD. The activity of SOD was measured and the content of protein was determined using coomassie brilliant blue G-250 method. The activity of SOD reached the maximum of 92.6% when the concentrations of Cu^(2+) and Zn^(2+) were 5.4?mmol/L and 3.6?mmol/L, respectively. Under the condition of 5.4?mmol/L Cu^(2+),3.6?mmol/L Zn^(2+) and heat-denaturalization at 75℃, the highter active SOD could be extracted. The experiment provided useful data for the development of a more simple and economical purification method.