创伤性休克大鼠重要脏器中一氧化氮与P-选择素的变化及意义

The Changes and Significance of Nitric Oxide and P-selectin in Vital Organs During Traumatic Shock in Rat

目的 探讨创伤性休克时重要脏器中一氧化氮与P 选择素的含量变化及其意义。方法 复制大鼠创伤性休克模型 ,采用镉还原比色法 (Griess)及酶联免疫法 (ELISA)测定休克后 30min、休克后 1h、复苏后 1h、复苏后 4h大鼠肝脏、小肠、肺脏、心脏组织中的NO-2 /NO-3 、P 选择素的含量。结果 正常大鼠大多数重要器官中的NO及P 选择素均含量较低 ,休克后 30min及 1h ,肝脏、小肠、肾脏、心脏中的NO较对照组下降 ,而P 选择素含量较对照组升高 ,但两者均无明显统计学意义 ;复苏后 1h ,肝脏、小肠、肺脏、心脏中NO下降明显而P 选择素含量显著升高 ;复苏后 4h ,肝脏、小肠、肺脏、心脏中NO及P 选择素含量显著升高。结论 创伤性休克时体内重要器官中的NO和P 选择素含量变化与休克时存在明显的微循环紊乱、内皮功能障碍。

Objective To investigate the changes and significance of nitric oxide and P-selectin in vital organs during traumatic shock in the rats.Methods The rats were subjected to traumatic shock model followed by measurement of the nitric oxide and P-selectin in the liver,intestine,lung and heart,by Griess and ELISA.Results The nitric oxide and P-selectin were low in almost all organs of normal rats.In 30 min and 60 min after shock,all the organs' nitric oxide were insignificantly down-regulated,but P-selectin were insignificantly increased than that in the control group.In 1 h after resuscitation,the nitric oxide was significantly down-regulated and the P-selectin was significantly increased.In 4 h after resuscitation,the nitric and P-selectin were significantly up-regulated.Conclusion The altertion of nitric oxide and P-selectin is related with a marked microcirculatory perturbation, endothelial dysfunction,reperfusion injury,and bacterial translocation during traumatic shock.