鸟嘌呤核苷高产菌的育种策略

The strategy for breeding of guanosine producting mutant

选育优良的鸟嘌呤核苷(鸟苷)产生菌是实现发酵法生产鸟苷的前提,在鸟苷发酵的建立和改进中起着非常重要的作用。本文分析了鸟苷的生物合成途径及代谢调节机制,根据代谢控制发酵“进、通、节、堵、出”五字原理,提出鸟苷高产菌的育种策略:鸟苷高产菌株在遗传学上应具备Ade-、red-、NP-、8-AGr(或8-AXr)、MSOr、SGr、AARr、Smr等遗传标记,并详述了其育种实例。

Breeding a high guanosine producting srain is the precondition of producting guanosine by fermentation, which was much of importance in designing and ameliorating the fermentation of guanosine. The metabolic pathway and regulation mechanism of guanosine were analyzed. On the principle of metabolic control fermentation, a high guanosine producting srain should be marked with Ade-, red-, NP-, 8-AGr(or 8-AXr), MSOr, SGr, AARr, Smr, etc. The strategy for breeding of guanosine producting strain and its instances were summarized comprehensively here.