人肝热缺血安全时限的基因芯片分析

Analysis of safe limit of human hepatic warm ischemic time by microarray

目的 探讨人肝不同缺血时限细胞凋亡相关基因的多基因表达模式和差异表达 ,为掌握肝缺血安全时限提供科学依据。方法 应用基因芯片表达谱技术观测正常人肝组织与肝缺血 15min(组一 )、30min(组二 )及肝缺血 15min与 30min(组三 )相比较细胞基因表达状况 ,对 2 0 0点特异性凋亡相关基因差异表达进行分析比较。结果 肝缺血 0～ 15min ,凋亡相关基因的表达基本处于低表达或正常表达水平 ,表达明显上调的的差异表达基因主要为调节细胞内环境稳定的基因 ;而至肝缺血 30min ,各种维系细胞和细胞器内环境稳定的调控基因表达水平明显下降 ,多种促凋亡发生的相关基因、诱导凋亡发生的核转录因子则明显活化、表达上调。结论 一般而言 ,人肝一次热缺血时限以尽量不超过

Objective To investigate different patterns of expression of multiple genes in human ischemic liver to provide evidence about safe limit of human hepatic ischemia. Methods The responses of cells to hepatic ischemia and hypoxia after hepatic ischemia for 15 and 30 min were analyzed by cDNA microarray representing 4000 different genes containing 200 apoptotic correlative genes. Results During the period of hepatic ischemia for 0-15 min, there were lower or normal expression levels of apoptotic correlative genes and the maintenance hemostatic genes were expressed significantly higher. However, the expression levels of maintenance hemostatic genes were down-regulated and the expression of many apoptotic correlative genes and nuclear transcription factors were activated and up-regulated. Conclusions The safe limit of human hepatic warm ischemic time is generally less than 30 min.