630nm半导体激光照射对小鼠S180腹水瘤细胞的影响

To Study the Influence of 630nm Semiconductor Laser Irradiation on Rat S180 Ascitic Tumor Cells without Photosensitizers

目的探讨 6 30nm半导体激光体外照射对S180腹水瘤肿瘤细胞的影响方法用输出功率 2W、不同功率密度 (10 0、2 0 0、2 5 0mW/cm2 )不同的照光时间 (2 0、15、10、5、3min)的半导体激光照射S180腹水瘤肿瘤细胞 ,照光后继续培养 2 4小时。观察照光前后不同时间段的细胞形态改变。用流式细胞技术测定照光前后S180腹水瘤肿瘤细胞的凋亡情况。结果 :1.实验组与对照组细胞在照光前后各时间段 (照光前、后即刻、培养 2 4小时 )的细胞形态相比较无明显改变。 2 .实验组与对照组细胞经流式细胞仪检测细胞凋亡情况无明显差异。结论 :单用光动力学治疗剂量的 6 30nm半导体激光对体外肿瘤细胞无明显杀伤作用 ,且不诱导S180腹水瘤肿瘤细胞发生凋亡。

Objective: To study the influence of 630nm semiconductor laser irradiation on rat ascetic tumor cells without photosensitizers.Methods: In this work, rat ascitic tumor cells(s180) were incubation with no photosensitizers.630nm light (total output 2W) was delivered to tumor cells at different fluence rate:100、200、250mW/cm 2 . According to each fluence rate ,S180 tumor cells were again divided into 5 groups, which were irradiated with various time periods:20、15、10、5、3min. In all cases, S180 tumor cells were taken photos. We the morphology and flow cytometry to investigate the apoptosis of tumor cells during the experiment. Results: 1、No obvious morphological difference between test groups and control groups at various time periods during the experiment.2、No obvious cell apoptosis difference between test groups and control groups using FCM.Conclusion: After irradiated with 630nm semiconductor laser light, rat ascitic tumor cells(s180) with no photosensitizers incubation were not obviously killed or induced apoptosis.