室内气传真菌代谢产物的细胞毒性研究

The cytotoxic effects of extracts from dominant indoor airborne fungal culture

目的 :观察研究室内常见气传真菌代谢产物的细胞毒性。 方法 :MTT比色法和细胞克隆形成率实验观察了室内常见气传真菌代谢产物对中国仓鼠肺细胞 (CHL)的存活和增值能力的影响 ,并通过检测细胞培养液中乳酸脱氢酶 (LDH)活力、胞内Ca2 + 、K+ 含量 ,观察真菌代谢产物对细胞膜通透性的影响。 结果 :随着室内常见气传真菌代谢产物剂量的上升 ,培养液中LDH相应上升 ,细胞内K+ 浓度降低 ,而细胞外的Ca2 + 有内流趋势。 结论 :常见气传真菌代谢产物能够损伤细胞膜 。

Objectine:To study the cytotoxicity of extracts of dominant indoor airborne fungal culture. Methods:Chinese hamster lung(CHL) cell culture was carried out in vitro.Cells were exposed to extracts of dominant indoor airborne fungal culture,then MTT method and CHL colonies assay were performed to detect their cytotoxicity.The release of cytosolic enzyme Lactate Dehydrogenase(LDH) and the fluidity of K^+,Ca^(2+) were analysed. Results:With the increase of the dose,the LDH levels in culture medium increased and the Ca^(2+) concentration inside cells increased while the K^+ concentration inside cells decreased accordingly. Conclusion:The extracts of dominant indoor airborne fungal culture could damage cell membrane,and have cytotoxicity.