细胞凋亡与宫颈癌新辅助化疗

The Relationship between Apoptosis and the Neoadjuvant Chemotherapy Response of Cervical Cancer

目的 :探讨细胞凋亡与宫颈癌新辅助化疗 (NACT)疗效的关系。方法 :2 0 0 0年 3月～ 2 0 0 4年 3月期间 ,4 7例Ib2 ～Ⅲa期原发性宫颈癌患者进行新辅助化疗 ,根据NACT近期临床疗效分为NACT有效组和无效组 ,用TUNEL法测定肿瘤组织的凋亡指数AI,观察两组NACT前后细胞凋亡水平的变化。结果 :NACT后AI明显高于NACT前 ,而有效组NACT前后的AI均高于无效组 ,有效组NACT后AI升高多于无效组 ,两者有显著差异 (P <0 .0 5 ) ,多因素结果表明 ,NACT前AI与NACT疗效有关 (P <0 .0 5 )。结论

Objective: To study the relationship between apoptosis and the neoadjuvant chemotherapy response of cervical cancer. Methods: From March 2000 to March 2004, 47 women with primary cervical cancer in stage Ib 2～Ⅲa according to the FIGO classification received NACT. They were divided into the effective group and non-effective group according to the response to NACT. In the specimens of pretreatment and post-operation, apoptotic index (AI) were evaluated by TUNEL method. And AI before and after NACT of the two groups was assayed. Results: The mean AI after NACT was significantly increased than that of the pretreatment cases (P<0.05). Either before or after NACT, the mean AI was significantly higher in effective cases (P<0.05). Multiple regression analysis suggested AI was correlated with the NACT response (P<0.05). Conclusion:AI is a useful parameter of the evaluation value of NACT response.