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脂肪分化相关蛋白基因与2型糖尿病相关 被引量:1

Adipose differentiation-related protein gene is associated with type 2 diabetes
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摘要 目的通过单核苷酸多态性(SNP)的检测及基因分型来研究脂肪分化相关蛋白基因(ADRP)与2型糖尿病的相关性。方法使用PCR测序方法,对ADRP基因的启动子、外显子以及临近的内含子,分别在正常人群、2型糖尿病人群进行测序,确定该基因是否与华东地区汉族2型糖尿病患者相关。结果所测ADRP基因片段总长度5003个碱基,有23个SNP,高频、低频分别是5个、18个。启动子的一个SNP(P/-407)在2型糖尿病人群与正常人群的等位基因型、基因型差异均有显著性(P<0.05);两个错义突变Ser251Pro、Thr396Ala分别在两个2型糖尿病家系中传递。结论ADRP可能与华东地区汉族人2型糖尿病相关。 Objective To investigate the association of adipose differentiation-related protein (ADRP) genewithtype2diabetesbysinglenucleotidepolymorphism(SNP) detection and genotyping. Methods Sequences of proximal promoters, coding regions (including sequence near splicing site) of ADRP gene was detected by PCR-sequencing in type 2 diabetics and normal controls, who were Chinese Hans residing in east and southeast China. Results The length of ADRP gene fragment assayed was 5003 bp and 23 SNPs were identified which included 5 high frequency SNPs and 18 low frequency SNPs. One SNP (P/-407) located in the promoter of ADRP had significant difference of allele and genotype distributions between type 2 diabetics and controls (both P<0.05). Two mis-sense mutations (Ser251Pro、Thr396Ala) were co-segregated with type 2 diabetes in two pedigrees. Conclusion The ADRP gene may associate with type 2 diabetics in eastern and southeastern Chinese Han population.
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2004年第4期304-306,共3页 Chinese Journal of Endocrinology and Metabolism
基金 国家科委973项目(G1998051017)
关键词 2型糖尿病 ADRP 相关蛋白 基因 正常人群 病人 分化 启动子 内含子 碱基 Adipose differentiation-related protein gene Ploymorphism, single nucleotide Diabetes mellitus, type 2
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参考文献7

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同被引文献14

  • 1袁中华,杨永宗,尹卫东,易光辉,王佐,杨保堂,万载阳,吴孟津.脂肪分化相关蛋白在兔动脉粥样硬化模型中的表达[J].中国动脉硬化杂志,2004,12(5):529-532. 被引量:2
  • 2赵小玲,刘益平,李亮,蒋小松,杜华锐,朱庆.脂肪分化相关蛋白基因多态性与优质鸡屠宰性状和肌内脂肪含量的相关性研究[J].遗传,2007,29(12):1483-1490. 被引量:7
  • 3T.H.Kim,B.H.Choi,G.W.Chang,K.T.Lee,H.Y.Lee,J.H.Lee,K.S.Kim,C.K.Park,C.Moran.??Molecular characterization and chromosomal mapping of porcine adipose differentiation‐related protein (ADRP)(J)Journal of Animal Breeding and Genetics . 2005 (4)
  • 4Chang Benny Hung-Junn,Chan Lawrence.Regulation of Triglyceride Metabolism. III. Emerging role of lipid droplet protein ADFP in health and disease. American journal of physiology. Gastrointestinal and liver physiology . 2006
  • 5Magnusson Bj?rn,Asp Lennart,Bostr?m Pontus,Ruiz Michel,Stillemark-Billton Pia,Lindén Daniel,Borén Jan,Olofsson Sven-Olof.Adipocyte differentiation-related protein promotes fatty acid storage in cytosolic triglycerides and inhibits secretion of very low-density lipoproteins. Arteriosclerosis . 2006
  • 6Subramanian Vidya,Rothenberg Alexis,Gomez Carlos,Cohen Alex W,Garcia Anne,Bhattacharyya Sucharita,Shapiro Lawrence,Dolios Georgia,Wang Rong,Lisanti Michael P,Brasaemle Dawn L.Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes. Journal of Biological Chemistry . 2004
  • 7Davoli Roberta,Gandolfi Greta,Braglia Silvia,Comella Marco,Zambonelli Paolo,Buttazzoni Luca,Russo Vincenzo.New SNP of the porcine perilipin 2 (PLIN2) gene, association with carcass traits and expression analysis in skeletal muscle. Molecular Biology . 2010
  • 8Jiang H P,Serrero G.Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein. Proceedings of the National Academy of Sciences of the United States of America . 1992
  • 9S. O. Shepherd,M. Cocks,K. D. Tipton,A. M. Ranasinghe,T. A. Barker,J. G. Burniston,A. J. M. Wagenmakers,C. S. Shaw.??Preferential utilization of perilipin 2‐associated intramuscular triglycerides during 1 h of moderate‐intensity endurance‐type exercise(J)Experimental Physiology . 2012 (8)
  • 10Larigauderie Guilhem,Furman Christophe,Jaye Michael,Lasselin Catherine,Copin Corinne,Fruchart Jean-Charles,Castro Graciela,Rouis Mustapha.Adipophilin enhances lipid accumulation and prevents lipid efflux from THP-1 macrophages: potential role in atherogenesis. Arteriosclerosis . 2004

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