磷酸三丁酯(TBP)的煤油溶液从发酵滤液中萃取青霉素的新工艺研究

A NEW PROCESS FOR THE EXTRACTION OF PENICILLIN G FROM THE FILTRATE OF FERMENTATIOM BROTH WITH TBP IN KEROSENE

本文研究了TBP的煤油溶液萃取青霉素的工艺,结果表明:在20～25%TBP-煤油为萃取剂,萃取相比O/A=1/3,平衡pH3.0～3.5;用1.5～2.0%NaHCO_3反萃,相比A/O=1/2,平衡pH 6.7～7.2,混合1分钟,醋酸钾水溶液结晶的工艺条件下,该工艺能循环运转,破乳剂用量仅为原工艺的四分之一,青霉素酸解破坏的减少将能保证提取收率的提高,但要获得优质产品还需对结晶工艺进行相应的研究、改造.

Penicillin G is a widely used antibiotic as well as the raw material for the semisynthetic penicillins. Penicillin G is a weak acid and is unstable in the aqueous solution, especially when the pH of the solution is not in the range of 6-8. The extraction is usually carried out at pH1.8-2.5 with butyl acetate or methyl isobutyl ketone(MiBK) as solvent in centrifugal extractors. The recorvery of the solvent extraction is about 90-91% from the filtrate of fermentation broth due to penicillin decomposition and other reasons(such as emulsion formation during extraction). This paper reports the possibility of increasing the extraction equilibrium pH and improving the demulsification by using TBP in kerosene as extractant to increase the recovery of penicillin G. The experimental results indicate that the TBP in kerosene can be used as the extractant for the extraction of penicillin G. The extraction equilibrium pH can be increased from 1.8-2.5 with butyl acetate used as solvent to pH3-4 due to the stronger extraction capability of the solvent of present choice. The recovery of penicillin G is increased with TBP in kerosene as solvent while only one-fourth of the demulsifier consumed. The crystallization process needs to be improved in order to obtain high quality penicillin G product.