摘要
目的探讨三七皂甙对大鼠脑缺血再灌注后基质金属蛋白酶-9(MMP-9)表达的影响。方法采用半定量RT—PCR测定MMP-9的变化,测定脑梗死体积并观察血脑屏障的破坏程度。结果再灌注后22 h,三七皂甙治疗组脑梗死体积和脑缺血区血脑屏障的破坏程度均低于对照组(P<0.05);三七皂甙治疗组MMP-9的表达明显低于对照组。结论三七皂甙能减轻脑缺血再灌注后的损伤和血脑屏障的破坏程度,抑制MMP-9的表达可能是其作用机制之一。
Objective To investigate the effect of Panax notoginseng saponins on metalloproteinase-9 ( MMP-9 ) expression after focal cerebral ischemia-reperfusion. -Methods Using intravascular thread model with 2 h occlusion and 22 h reperfusion in rats, the brain infarction size and the degree of blood-brain barrier ( BBB) disruption in the ischemic region were evaluated. MMP-9 expression was evaluated by semi-quantitative RT-PCR. Results The brain infarction volume and area of BBB disruption were dramatically decreased in treatment group as compared to control group. The level of MMP-9 mRNA in experimental group was significantly lower than control rats. Conclusion BBB disruption and ischemic injury were attenuated by Panax notoginseng saponins after focal cerebral ischemia-reperfusion, one mechanism is through its inhibition of MMP-9 expression.
出处
《上海第二医科大学学报》
CSCD
2004年第9期731-733,共3页
Acta Universitatis Medicinalis Secondae Shanghai
