肝癌患者血清及组织中ROS、T-SOD、MnSOD水平的测定

Determination of reactive oxygen species,total superoxide dismutase and manganese superoxide dismutase in serum and tissue of primary hepatocellular carcinoma patients

目的 研究原发性肝癌患者血清及组织内活性氧类物质 (ROS)、总超氧化物歧化酶 (T SOD)、锰超氧化物歧化酶 (MnSOD)水平。方法 选择 2 0例原发性肝癌组织和癌旁组织及对应血清。采用Fenton反应测定血清中ROS ,组织内ROS测定采用流式细胞技术。患者血清及组织匀浆内T SOD、MnSOD采用黄嘌呤氧化酶法测定。结果 原发性肝癌患者血清和组织内ROS水平明显高于正常对照 (P <0 .0 1) ,肿瘤患者血清中T SOD和MnSOD水平低于正常对照 (P <0 .0 1) ,血清中ROS与MnSOD呈明显负相关 (r =- 0 .92 4 ) ,癌组织内T SOD和MnSOD水平明显高于癌旁组织 (P <0 .0 1)。结论 ROS可能参与肝癌的发生 ,MnSOD在消除ROS阻止肝癌发生中可能发挥重要作用。血清中ROS、MnSOD与组织ROS、MnSOD之间可能有重新分配的过程。

Objective To study the serum and tissue levels of reactive oxygen species(ROS),total superoxide dismutase(T-SOD) and manganese superoxide dismutase(MnSOD) in patiens with primary hepatocellular carcinoma(PHC). Methods Malignant and adjacent normal tissues and serum sample were collected from 20 PHC patients. Serum ROS and tissue ROS were detected by Fenton reaction and flow cytometry respectively. Serum and tissue T-SOD and MnSOD were detected by xanthine oxidase method. Results ROS levels of PHC patient were much higher than the control group(P<0.01). Serum T-SOD,MnSOD levels of PHC patient were much lower than the control group(P<0.01). In serum the ROS level showed negative correlation with the MnSOD level(r=-0.924). The tissue T-SOD,MnSOD levels of PHC patient were much higher than the control group(P<0.01). Conclusions ROS and MnSOD may play an important role in the process of hepatocarcinogenesis.