摘要
目的 研究中国汉族人群中血清肿瘤坏死因子α(TNFα)水平及其 - 2 38、- 30 8位点基因多态性与冠心病之间的相关性。方法 采用双抗体夹心酶联免疫吸附试验 (ELISA)检测血清TNFα水平 ,同时应用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)技术检测TNFα基因多态性。结果 冠心病组血清TNFα水平显著高于对照组 (P <0 .0 5 )。两组的 - 2 38、- 30 8位点基因型和等位基因的分布差异无显著性 (P >0 .0 5 ) ,- 2 38位点GA +AA基因型的血清TNFα水平 [(2 3.5 7± 6 .96 )ng/L]显著高于GG基因型 [(17.2 8± 7.17)ng/L](P <0 .0 5 ) ,在陈旧性心肌梗死 (OMI)患者和稳定型心绞痛 (SA)患者中均未检出GA和AA基因型 ,但与急性心肌梗死 (AMI)患者及不稳定型心绞痛 (UA)患者比较 ,差异无显著性 (P >0 .0 5 )。结论 血清TNFα水平显著升高提示炎性反应在冠心病病程中有重要作用 ;TNFα的水平可能受其基因多态性的影响。
Objective To investigate the relationship between plasma levels of tumor necrosis factor α(TNFα) or its -238,-308 sites polymorphism and coronary heart disease (CHD) in Chinese. Methods Plasma level of TNFα was measured by ELISA,and the polymorphism of TNFα gene was screened by PCR-RELP. Results CHD group showed significantly higher plasma level of TNFα than control group (P<0.05). There was no significant difference between CHD group and control group for TNFα-238,-308 sites polymorphism (P>0.05). Plasma TNFα level was significantly higher among carriers of -238A allele as compared with non-carriers (GG genotype)[(23.57± 6.96 ) vs (17.28±7.17) ng/L,P<0.05]. There was no significant difference in the number of old myocardial infarction (OMI),acute myocardial infarction(AMI),unstable angina pectoris(UA) and stable angina pectoris (SA) by TNFα-238 polymorphism (P>0.05). Conclusions Plasma TNFα increases in CHD and there is no association between the -238,-308 polymorphisms and CHD in Chinese.
出处
《检验医学》
CAS
北大核心
2004年第5期434-437,共4页
Laboratory Medicine
基金
湖北省自然科学基金资助项目 ( 2 0 0 3ABA183 )
