血浆凝血酶原时间测定中建立仪器区域国际敏感指数的实验探讨

Establishment of local international sensitivity index of instrument in the prothrombin

目的 探讨临床口服抗凝治疗时血浆凝血酶原时间 (PT)监测的标准化 ,为临床提供比较准确的PT 国际标准化比值 (PT INR)结果。方法 使用PT INR校准血浆建立PT测定试剂在不同仪器上的区域性国际敏感度指数 (LocalISI) ,以此对新鲜血浆进行PT测定。结果 未经LocalISI校准的PT试剂测定血浆的PT时 ,INR结果差异较大 (P <0 .0 1)。试剂经LocalISI校准后 ,测定血浆PT时 ,INR结果良好 (P >0 .0 5及Kap pa >0 .75 )。 结论 在PT测定时 ,只要建立仪器和测定试剂的LocalISI ,INR结果就具有较好的一致性。

Objective To investigate the standardization on the prothrombin time-international normalized ratio (PT-INR) determination,it will be great help to provide more accurate testing results on clinical anti-coagulation therapy. Methods Using PT-INR calibrated plasma to establish PT-INR determining reagents' local international sensitivity index (ISI) among different instruments for the determination of PT in fresh plasma. Results When plasma PT was determined after using marked ISI reagents,the INR results had significant difference (P<0.01). When plasma PT was determined after using local ISI,the INR results were satisfied (P>0.05, Kappa>0.75). Conclusions For the consistency of the final results in the determination of plasma PT,it's necessary to set up local ISI for related instrument and reagent.