马齿苋对D-半乳糖衰老模型小鼠应激能力的影响

Effects of purslane herb on stress ability of aging mice induced by D-galactose

目的:观察马齿苋水提液对老年动物应激能力的影响并探讨其机制。方法:以维生素E为对照,观察不同浓度马齿苋水提液对D-半乳糖衰老模型小鼠缺氧存活时间、爬杆时间、高温存活率的影响。采用荧光法测定脑和肝脏脂褐质、丙二醛(ma-londialdehyde,MDA)含量;采用羟胺法测定脑、肝超氧化物歧化酶(superoxidedismutase,SOD)活性;采用钼酸胺显色法测定过氧化氢酶(catalase,CAT)活性。结果:与模型组相比:(1)1.6、0.8、0.4ml／d3种浓度的马齿苋水提液可明显延长模型小鼠的缺氧生存时间、爬杆时间,提高其高温存活率,其中以中剂量组效果最好;(2)灌喂0.8ml／d马齿苋组小鼠的SOD和CAT活性下降的幅度明显减小,肝、脑细胞内的脂褐质和MDA含量明显减少;(3)维生素E虽然对抗氧化酶也有一定的保护作用,但效果不如马齿苋水提液。结论:马齿苋水提液可保护衰老模型小鼠的应激能力,机制之一可能是其促进了SOD和CAT等抗氧化酶活性,减轻了氧化产物对机体的损伤。

Objective: To investigate the effects of purslane herb aquenous extracts (PHAS) on the stress ability of aging mice induced by D-galactose. Methods:We odserved the survival time to hypoxia and heat survival rate of the mice treated with different doses of PHAS and vitamin E. The contents of lipofuscin and malondialdehyde (MDA) , and the activity of superoxide dismutase (SOD) and catalase (CAT) in the brain and liver of the mice were tested. Results: As compared with vitamin E, three doses of PHAS (1. 6, 0. 8 and 0. 4 ml/d) prolonged the survival time to hypoxia and the pole climbing time and increased the heat survival rate, and the 0. 8 ml/d PHAS had the best effect. In the group of 0. 8 ml/d PHAS, the activity of SOD and CAT decreased less, and the contents of lipofuscin and MDA decreased significantly. The effect of vitamin E was not as good as the PHAS. Conclusion:PHAS can prevent the stress ability of the aging mice. One of its mechanisms may be increasing the activity of SOD and CAT, hence decreasing the damage of the oxidation products to the body.