山刺玫果抗脂质过氧化作用的实验研究

Anti-Hpid peroxidation effect of Rosa davurica Pall. fruit

目的:探讨山刺枚果抗脂质过氧化的作用.方法:测试山刺玫果提取液在体外抗小鼠肝、心、肾、脑组织匀浆的脂质过氧化作用及其抗H2O2引发的红细胞的脂质过氧化作用,并观察山刺玫果提取液在体内对小鼠缺血心肌丙二醛(malondialdehyde,MDA)含量和超氧化物岐化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性的影响.结果:山刺玫果0.4 g/L提取液组体外抑制心、肝、肾、脑组织MDA生成的抑制率为77.1%～87.5%,0.2 g/L提取液组为17.3%～33.4%.山刺玫果提取液在浓度分别为6.7、3.3、1.7 g/L时,对H2O2引起红细胞MDA生成的阻断率分别为89.2%、53.4%、26.6%.小鼠灌胃山刺枚果2.0 g·kg-1·d1,连续6 d,可显著降低缺血心肌MDA的含量(P 《0.01),提高GSH-Px和SOD活性(P 《0.05);而4.0 g·kg-1·d-1剂量作用更显著(P《0.01).结论:山刺玫果具有显著的抗脂质过氧化作用,其抑制强度与剂量呈正比.

Objective: To study the anti-lipid peroxidation action of Rosa davurica Pall. fruit. Methods: The contents of malon-dialdehyde(MDA) of the tissue homogenate of the liver, heart, kidney, brain and of the red blood cells induced by hydrogen peroxide in mice were measured. The contents of MDA and the activities of superoxide dismutase (SOD) and glutathione per-oxidase (GSH-Px) of ischemic myocardium of mice were measured. Results: 0. 2 g/L Rosa davurica Pall. fruit could decrease significantly the contents of MDA of the all tissue( P <0. 05). Inhibition rate of 6. 7 g/L Rosa davurica Pall. fruit on MDA of the red blood cells induced by hydrogen peroxide was 89. 2%. Administration of this extraction successively for six days (ig, 2. 0 g · kg-1 · d-1) can significantly reduce the content of MDA ( P <0. 01) and augment the activities of SOD and GHS-Px ( P <0. 05) in the ischemic myocardium of mice. Conclusion: Rosa davurica Pall. fruit can significantly prevent the lipid peroxidation.