益气化瘀方对大鼠腰神经根损伤后花生凝集素结合分子和施万细胞的作用

Effect of Yiqi Huayu Recipe on peanut agglutinin-binding molecules and Schwann' s cells in rats after lumbar nerve root compression

目的:研究大鼠L5神经根损伤后,益气化瘀方对神经肌肉接头部花生凝集素结合分子(peanutagglutinin-bindingmolecules,PNA-BMs)和施万细胞(Schwann'scell,Sc)的作用。方法:大鼠L5神经根损伤后,给与益气化瘀方治疗。于用药后第10、20、30、60天,采用免疫组织化学和激光共聚焦扫描显微镜技术。观察PNA-BMs及作为Sc标记的S-100在大鼠比目鱼肌中的分布。使用NIH图像分析技术,定量测定PNA-BMs与S-100的重叠面积。结果:被PNA识别的细胞外基质(extra-cellularmatrix,ECM)在Sc延伸过程中起着重要的导向作用。益气化瘀方对Sc在神经肌肉接头部的聚集、出芽、延伸,以及与PNA-BMs的重叠面积均明显优于对照组。结论:益气化瘀方能明显促进ECM和Sc的生长,从而加速神经再生修复进程。

Objective: To study the effect of Yiqi Huayu Recipe on peanut agglutinin-binding molecules (PNA-BMs) and Schwann's cells (Sc) of the neuromuscular junction after L5 nerve root compression in rats. Methods:The rats were given Yiqi Huayu Recipe at the 10th, 20th, 30th and 60th day after L5 nerve root compression. Using immunohistochemistry and confocal laser scanning techniques, we observed the distributions of PNA-BMs and S-100 in the soleus muscle. The overlap area of PNA-BMs and S-100 was measured with NIH image technique. Results: The extracellular matrix (ECM) recognized by PNA played an important role in guiding the sprouting and extending of the Schwann's cells. The agglomeration, sprouting and extension of the terminal Sc and the overlap area of Sc with PNA-BMs in the Yiqi Huayu Recipe group were better than those in the control group. Conclusion: Yiqi Huayu Recipe can promote the growth of ECM and Schwann's cells and accelerate the nerve regeneration process.