肠缺血再灌注损伤对血清及脂肪组织Leptin水平的影响(英文)

Effect of intestinal ischemia reperfusion injury on the leptin levels in serum and adipose tissue

背景:近年来的研究发现脂肪组织不仅仅是脂肪的储存库,还是一种能够分泌多种功能性细胞因子的多潜能内分泌器官。Leptin是一种由脂肪组织特异性分泌的蛋白质,主要功能是抑制摄食和增加能量消耗。目的:研究肠缺血再灌注损伤对血清及脂肪组织水平的影响,探Leptin讨Leptin在急性炎症反应中的作用。设计:完全随机设计,自身及组间对照。地点和材料:本实验于解放军总医院生化研究室完成。3只雄性新西兰白兔及54只雄性SD大鼠作为实验动物。干预:免疫新西兰白兔获得Leptin抗体,采用氯胺-T法碘标记Leptin抗原,由此建立一种简便的鼠Leptin放射免疫分析法。建立大鼠肠缺血再灌注损伤模型,实验大鼠随机分为组:假手术组(sham、缺血6)60min-再灌注30min组(I60-R),30I60-R90,I60-R150,I60-R240及I60-R360组,每组9只。主要观察指标:采用放射免疫分析法测量血清及脂肪组织Leptin浓度变化。结果:与损伤前自身对照组的血清Leptin水平相比,肠缺血60min再灌注损伤30min(I60-R30)组显著降低(t=2.3891,P<0.05),I60-R150组呈现增高的趋势,而I60-R360组显著增高t=-2.3437,P<0.05);与损伤后(组的血清水平shamLeptin(9.88±1.87)μg/L相比,I60-R240组呈现增高的趋势,而I60-R360组(19.43±2.84)μg/L显著增高(t=-2.8085,P<0.05);

BACKGROUND:Recent studies suggest that adipose tissue is not only the reservation of fat,but also a multi potential endocrine organ secreting many functional cytokines.Leptin is a protein specifically secreted by adipose tissue,and primarily reduces food intake and promotes energy expenditure. OBJECTIVE:To explore the effect of intestinal ischemia reperfusion injury on leptin levels in serum and adipose tissue,and find out the role of leptin playing in acute inflammatory responses. DESIGN:A completely random self and mutual control experiment. SETTING and MATERIALS:The experiment was collected and completed in Research Laboratory of Biochemistry,General Hospital of Chinese PLA. Three male New Zealand white rabbits and fifty four male Sprague Dawley rats were used for experimental use. INTERVENTIONS:New Zealand white rabbits were immunized to obtain anti leptin,leptin antigen was iodinated by chloramines T method,thus we established a concise radioimmunoassay for murine leptin.An intestinal ischemia reperfusion injury model of rats was established,and rats were divided randomly into six groups:sham operation group(sham),60 minute ischemia and 30 minute reperfusion group(I60 R30),I60 R90,I60 R150,I60 R240 and I60 R360;each group contained nine rats. MAIN OUTCOME MEASURES:The changes of leptin concentrations in serum and adipose tissue before and after ischemia reperfusion injury were checked by the radioimmunoassay. RESULTS:Compared with self control(before injury),serum leptin level of I60 R30 decreased significantly(t=2.3891, P< 0.05),that of I60 R150 expressed a trend to increase and that of I60 R360 increased significantly(t=-2.3437,P< 0.05).Compared with sham after injury [(9.88±1.87) μg/L],serum leptin level of I60 R240 expressed a trend to increase, that of I60 R360[(19.43±2.84) μg/L] increased significantly(t=-2.8085,P < 0.05).Compared with sham after injury[(11.12±1.27) ng],adipose leptin levels of I60 R30[(4.46±2.63) ng] and I60 R90[(3.45±2.77) ng] decreased significantly(t=2.2804 and 2.5170, respectively, both P< 0.05),while that of I60 R360[(17.54 ±1.87)ng] increased significantly(t=-2.8401,P< 0.05). CONCLUSION:Leptin has a time dependent response to acute inflammatory stimuli and participates in the energy metabolic disorders of acute inflammation.