瘦素促进移植颗粒脂肪成活的实验研究

Leptin as a survival enhanced-factor in grainy fat transplantation

目的 探讨瘦素是否具有促进移植颗粒脂肪组织成活的作用。方法 取大鼠腹部皮下脂肪组织制成颗粒脂肪 ,分别用NS及 2 0ng ml瘦素处理后行自体头皮下移植。在不同观察时间取出移植组织称重 ,同时行HE及血管内皮细胞生长因子 (VEGF)染色。实验结果用SPSS统计分析软件进行统计学分析。结果 实验组和对照组比较 ,用瘦素处理的组织其周围包膜薄 ,脂肪细胞坏死融合少 ,重量维持率在移植 10、2 0、4 0d后分别为 10 8 3%、83 3%、6 6 3% ,和NS处理相应时间组6 0 0 %、4 2 1%、39 5 %的重量维持率相比 ,差异有显著性意义 (P <0 0 5 )。移植组织内VEGF染色强度较高。结论 瘦素对移植鼠颗粒脂肪组织具有促进成活作用 ,其作用机理可能是促进移植组织的血管增生。

Objective This experiment is to test the survival enhance effect of leptin in grainy fat transplantation. Methods The 0.6 g grainy fat was obtained from the Wastar mouse subcutaneous tissue in the abdomen. It was then mixed with 0.9%NS and 20 ng/ml leptin and transplanted in the head subcutaneously. The transplanted tissue was taken out after 10,20 and 40 days. After being weighted,the tissue was dyed with HE staining and VEGF staining. The data was analyzed with SPSS software. Results The fibrous membrane around the tissue in leptin group was thin and there was less fat cell necrosis,compared with the control group. The remained-weight rate was 108.3%,83.3% and 66.3% in 10,20 and 40 days after the surgery. The differences were statistic significant between the leptin groups and the control ( P <0.05). The examinations of the HE and VEGF stain showed that there was the rich vascularization in leptin treated tissue. Conclusion Leptin could enchance the survival of the transplanted grainy fat due to its angiogenesis effect. ;