芦荟苦素与熊果苷对体外培养的黑素细胞协同影响的实验研究

The effects of aloesin and arbutin on cultured melanocytes in a synergetic method

目的 研究芦荟苦素与熊果苷协同对体外培养的正常人表皮黑素细胞的效应。方法 在体外建立正常人表皮黑素细胞培养系 ,通过MTT法测定芦荟苦素与熊果苷的混合物对黑素细胞活力的影响 ,以左旋多巴为底物测定酪氨酸酶活性 ,用图像信号采集与分析系统测定黑素含量 ,并与单纯芦荟苦素组及熊果苷组进行比较。结果 芦荟苦素与熊果苷的混合物对体外培养的正常人黑素细胞酪氨酸酶活性有明显抑制作用 ,可显著减少黑素生成量 ,与单一药物对照组相比 ,差异有显著性意义 (P <0 0 5 ) ,而对黑素细胞活力影响很小 ,与单一药物对照组相比 ,差异无显著性意义。结论 芦荟苦素与熊果苷的混合物对体外培养的黑素细胞的酪氨酸酶活性及黑素生成有显著抑制作用 ,并较单一药物作用增强 ,说明两者呈协同作用。

Objective To study the effects of aloesin and arbutin on normal cultured human melanocytes in synergetic method. Methods Building up the system of cultured human melanocytes .The cultured melanocytes in vitro were treated with the mixture of aloesin and arbutin. The cell viability and tyrosinase activity was measured by MTT assay,utilization of L-Dopa as the substrate respectively;melanin content was measured by image analysis system. Furthermore,the effects of the mixture on melanocytes were compared with that of aloesin and arbutin. Results The mixture of aloesin and arbutin showed an inhibition on tyrosinase activity of human melanocytes and reduced significantly melanin content .Between the mixture and the single use of aloesin or arbutin,there is significant difference( P <0.05). On the other hand,the mixture has little influence on melanocytes viability and there is negative significance. Conclusion The mixture of aloesin and arbutin can significantly inhibit the tyrosinase activity and melanogenesis of cultured human melanocytes. It showed the effects of aloesin and arbutin in a synergistic manner . It is worth to give farther study later. ;