高效液相色谱-蒸发光散射检测法测定脑脉康注射液中黄芪甲苷的含量

HPLC - ELSD Determination of Astragaloside Ⅳ in Chinese Traditional Medicine Naomaikang Injection

目的:建立高效液相色谱-蒸发光散射检测法测定脑脉康注射液中黄芪甲苷的含量。方法:采用Lichrospher—C18(5μm,150 mm×6.0 mm)色谱柱,以甲醇-水(8:2)为流动相;流速:1.0 mL·min-1;气化室温度:100℃;N2气体流速:2.27 L·min～1。结果:黄芪甲苷在40.8—163.3μg·mL-1范围内呈良好的线性关系,r=0.9997;平均加样回收率为97.24%,RSD为1.2%。结论:本法操作简便,易行,重复性好,灵敏度高,可有效控制脑脉康注射液制剂中黄芪甲苷的内在质量。

Objective:To develop an HPLC - ELSD method for determination of astragaloside Ⅳ in complex preparation of Chinese Naomaikang injection. Methods:This method used Lichrospher - C18(5 μm,150 mm ×6. 0 mm) with methanol - water(8:2) as the mobile phase,1.0 mL · min-1 as flow rate,100 ℃ as inlet heater,2. 27 L · min-1 as N2 flow rate. Results:The linear ranges was 40. 8 -163. 3 μg · mL-1 for astragaloside Ⅳ with correlation coefficient at 0. 9997. The mean recovery was 97. 24% (RSD = 1. 2% ). Conclusions:This method is simple,reproducible , good sensitive and it can be used for the quality control of astragaloside IV in Naomaikang injetion.