HPLC法测定獐牙菜及其近缘植物中4种有效成分的含量

Simultaneous Determination of Four Effective Components in Swertia and Its Related Plants by HPLC

目的:建立反相高效液相色谱法同时测定獐牙菜及其近缘植物中番木鳖酸、獐牙菜苦苷、龙胆苦苷、芒果苷的含量。方法:采用ZORBAX SB—C18(250 mm×4.6mm,5μm)色谱柱,以流动相甲醇和水(含0.04%磷酸)的比例在0-24 min内由22:78至38:62线性梯度洗脱,流速1 mL·min～1,检测波长254 nm,柱温30℃。结果:4种成分均达到基线分离,番木鳖酸、獐牙菜苦苷、龙胆苦苷、芒果苷的线性范围分别为0.05—6.25μg(r=0.9999),0.0095—2.9 μg(r=0.9998),0.0486—2.56μg(r=0.9999),0.0056—2.8μg(r=0.9998);回收率为102%(RSD=4.4%),97.7%(RSD=4.3%),99.5%(RSD=3.5%),103%(RSD=1.1%)。结论:方法测定快速,结果准确、可靠。

Objective: To establish a quantitative method of simultaneously determination of loganic acid, swer-tiamarin, gentiopicroside and mangiferin in Swertia and its related plants by RP - HPLC. Method; The sample were separated on the column of Zorbax SB - C18 (250 mm * 4. 6 mm, 5 ?m) which eluted with methanol and water (0. 04% phosphoric acid). The ratio of methanol and water increased from 22:78 to 38:62 in 24 min with detected wavelength at 254 nm.flow rate at 1 mL · min-1,column temperature at 30 ℃. Results:Four compounds were base - isolated. The linear ranges of loganic acid, swertiamarin, gentiopicroside and mangiferin were 0. 05 - 6. 25 μg( r = 0.9999) ,0.0095-2.9 μg(r=0.9998) ,0.0486 -2.56 μg(r=0.9999) ,0.0056-2.8 μg(r=0.9998) respectively ;The average recoveries were 102% (RSD =4.4% ) ,97. 7% (RSD =4. 3% ) ,99. 5% (RSD = 3. 5% ), 103% (RSD = 1.1% )respectively. Conclusions:The method is rapid and precise.