人透明带蛋白3重组表达载体的构建与鉴定

Construction and identification of recombinant human zona pellucida 3 expression vector

目的 :构建高效表达人透明带蛋白 3的真核重组表达载体。方法 :利用 PCR、T- A载体克隆和亚克隆等技术。结果 :构建成 p GEM- ZP3重组质粒及 pc DNA3.1ZP3dhfr真核重组表达载体。结论 :经酶切鉴定构建的人透明带蛋白 3重组表达载体可高效表达重组人 ZP3蛋白。

Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used. Results The pGEM-TZP3 plasmid and pcDNA3.1ZP3dhfr recombinant expression vector were successfully constructed. Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.