诱导型一氧化氮合酶在肺癌组织中的表达及其与血管生成的关系

Expression of inducible nitric oxide synthase in lung carcinoma and its relation with angiogenesis

目的 :研究人体肺癌组织诱导型一氧化氮合酶 (i NOS)的表达及其与临床参数和肿瘤血管生成的关系。方法 :采用免疫组织化学及原位分子杂交技术检测 4 1例人体肺癌组织中 i NOS的表达情况 ,CD34染色显示肿瘤内微血管密度 (MVD) ,显微镜下观察计数。结果 :肺癌组织中有 i NOS m RNA和蛋白质表达 ,阳性率分别为6 0 .98%、 5 8.5 4 % ,明显高于癌旁及正常组织 ,阳性细胞主要为肿瘤细胞和间质巨噬细胞、中性粒细胞 ,i NOS表达与肿瘤临床分期及淋巴结转移有关 ,与 MVD值明显相关。结论 :肺癌组织中 i NOS的表达对术前评价患者淋巴结状态和临床分期中可能有一定价值 ,i NOS可促进血管生成 ,因此抑制 i NOS可望成为抗肿瘤血管生成疗法的又一新靶点。

Objective To study the expression of inducible nitric oxide synthase(iNOS) in lung carcinoma and its relation with clinical parameters and tumor angiogenesis. Methods The expressions of iNOS mRNA, protein and tumor interstitial MVD were detected in 41 lung carcinoma tissues by the method of in situ hybridization and immunohistochemistry.Results The expressions of iNOS mRNA and protein in lung carcinoma was significantly higher than that of peri-carcinoma and normal tissues(P<0.005). The positive rates were 60.98% and 58.54%, respectively. The positive staining was mostly localized in cytoplasm of tumor cells and some macrophages and neutrophils. There were positive correlations between iNOS expression and clinical stages and lymph node metastasis, and the MVD of iNOS positive group was obviously higher than that of negative group significantly. Conclusion The iNOS expression in lung carcinoma may be valuable in assessing lymph node metastasis and clinical stages for patients before operation. As the iNOS is able to promote angiogenesis, the inhibition of iNOS may become a new target for anti-angiogenic therapy.