TNP-470和MMC联合应用对鼠结肠癌生长和肝转移的作用

Effects of combined treatment with TNP-470 and MMC on growth and liver metastasis of mouse colon cancer

目的 :研究 O- (氨乙酰 -氨甲酰基 )烟曲霉醇 (TNP- 4 70 )和丝裂霉素 (MMC)联合应用对结肠癌生长和肝转移的抑制作用。方法 :建立裸鼠结肠癌的原位生长和肝转移模型 ,随机分为对照组、TNP- 4 70组、MMC组及联合用药组 (TNP- 4 70 +MMC)。结果 :4个组瘤重分别为 (4 99± 2 4 7)、 (4 78± 180 )、 (2 75± 80 )和(2 5 5± 6 5 ) mg,肝转移率分别为 87.5 %、 2 5 .0 %、 75 .0 %和 12 .5 % ,与对照组比较联合用药组抑制作用最明显(P<0 .0 1) ,且治疗后各组裸鼠活动良好 ,无明显体重减轻。结论 :TNP- 4 70与 MMC联合应用对结肠癌的生长及肝转移的抑制有协同作用 ,且未加重 TNP- 4 70引起的体重减轻等毒副作用。

Objective To study the effects of the combination of angiogenesis inhibitor TNP-470 and MMC on the growth and liver metastasis of colon carcinoma. Methods Metastatic model simulating human colon cancer M116 was established and the thirty-two nude mice were randomly divided into 4 groups: control group,TNP-470 group,MMC group,and TNP-470+MMC group. Results The average tumor weights in four groups were (499±247), (478±180), (275±80),and (255±65) mg, respectively. The liver metastasis rates in four groups were 87.5%, 25.0%, 75.0%, and 12.5%, respectively. Compared with control group, tumor growth was significantly inhibited by TNP-470+MMC, and the mice in each group showed no evident weight loss and no abnormal activities. Conclusion The combination of TNP-470 and MMC can produce additive effects on the growth and liver metastasis of colon cancer. The toxic action of TNP-470 is not affected by MMC.