癫痫大鼠海马结构谷氨酸和nNOS神经元的动态变化

Dynamic changes of glutamate and nNOS neurons in hippocampal formation in epilepstic rat

目的 :探讨谷氨酸 (Glu)和一氧化氮 (NO)二者在癫痫模型中的作用及其相关性。方法 :戊四唑化学点燃癫痫大鼠 ,分为Ⅰ、Ⅲ、Ⅴ级组和Ⅴ级后 2 4h组。采用免疫组织化学方法和图像分析技术。结果 :( 1 )Ⅲ、Ⅴ级组Glu免疫反应阳性神经元数目和平均光密度值升高 ;但Ⅴ级比Ⅲ级组有所下降 ;Ⅴ级后 2 4h组恢复到对照组水平。 ( 2 )Ⅴ级组和Ⅴ级后 2 4h组nNOS免疫反应阳性神经元数目和平均光密度值升高。结论 :戊四唑点燃癫痫模型中 ,随着点燃级别的进展 ,Glu的含量是呈先增加后减少的趋势 ,而NO的含量是逐渐增加的 。

Objective: To explore the role of glutamate (Glu) and NO in the PTZ kindling model. Methods: PTZ-kindling was used to build chronic epilepsy model. The rats were divided into control group, Grade-Ⅰ group, Grade-Ⅲ group, Grade-Ⅴ group and post-Grade-Ⅴ 24 h group. With immunohistochemical method and the image analyzing system, the changes of Glu and NOS-IR postitive cells in the hippocampal formation were observed at each stage. Results: (1) In the epileptic rats induced by PTZ-kindling, the numbers of Glu-IR positive cells and AOD of the Grade-Ⅲ group and Grade-Ⅴ group were increased compared with control group. Compared with Grade-Ⅲ group, the numbers of the Ⅴ-Grade goup were decreased. (2) After PTZ injection, the numbers of nNOS-IR positive cells and AOD of the Ⅴ-Grade group and post-Ⅴ-Grade 24 h group were increased compared with control group. Conclusion: As the progress of kindling, the express of Glu-IR increases first and then decreases gradually; the expression of NOS-IR increases gradually, suggesting both Glu and NOS are related to the epilepsy by PTZ kindling.