SLE患者血管内皮炎性因子的检测及临床意义

The detection and clinical significance of the blood vessel inflamed factors in systemic lupus erythematous

目的 :探讨患者血浆中血栓调节蛋白 (TM ) ,血清中性粒细胞胞浆抗体 (ANCA )及抗心磷脂抗体 (ACL)在系统性红斑狼疮 (SLE)中的临床意义。方法 :用ELISA法检测 86例SLE患者和 2 4例正常人血浆TM和血清中性粒细胞胞浆抗体 (包括P ANCA和C ANCA )及抗心磷脂 (包括ACL IgM和ACL IgG )抗体水平。结果 :( 1)SLE活动组TM ( 8 0 5 9± 2 13 3 )ng/mL水平均高于稳定期组 ( 4 15 4± 0 70 5 )ng/mL和对照组 ( 4 719± 0 691)ng/mL ,差异有统计学意义 (P <0 0 1)。SLE稳定期与对照组TM水平差异无显著性 (P >0 0 5 )。 ( 2 )SLE活动组ANCA (阳性率2 2 % )及ACL(阳性率 3 2 % )阳性率高于SLE稳定期ANCA(阳性率 13 % )和ACL(阳性率 2 4% )及对照组 (全阴 ) ,差异有显著意义 (P <0 0 1)。稳定期组ANCA和ACL阳性率与对照组 (全阴性 )差异有统计学意义 (P <0 0 5 )。结论 :患者血浆sTM、血清ANCA及ACL水平与SLE活动性有较好的相关性 ,可作为判断活动性及治疗效果的指标 。

Objective To observe the clinical si g nificance of serum thrombomodulin(TM), antineutrophil cytoplasmic autoantibodies (ANCA)and anti-cardiolipin antibody(ACL) in systemic lupus erythematosus(SLE) . Methods TM, ANCA(P-ANCA and C-ANCA)and ACL(ACL-IgM and ACL-IgG)were tested in 24 healthy individuals and 86 SLE cases with ELISA. Results (1)The active SLE group: the concentrations of TM(8 .059±2.133)ng/mL were higher than inactive SLE(4.154±0.705)ng/mL and co ntrol group(4.719±0.691)ng/mL. The differences had statistical significance (P<0.01). The concentrations of TM between inactive SLE group and control group hadn't si gnificant difference(P>0.05). (2)The rate of ANCA-positive(22%) and ACL-p oitive(32%) in active SLE group had higer than ANCA-positive(13%) and ACL-poit i ve(24%) in inactive SLE group and control group(all negative). The differences h ad statistical significance(P<0.01). The differences of ANCA-positive and ACL-poitive between inactive SLE group and control group had significance(P <0.05). Conclusion The concentrations of TM, ANCA and ACL in SLE were related with the activity, which can be used to decide activity and treatm ent result as marker and also can be uesed as warns for vessel trouble in SLE.