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3,4亚甲基二氧基甲基苯丙胺神经毒性的实验研究 被引量:5

Neurotoxicity of 3,4-methylenedioxy methamphetamine
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摘要 目的:探讨3,4亚甲基二氧基甲基苯丙胺(3,4-methylenedioxymetham-phetamine,MDMA)所致实验大鼠的神经毒性及胶质纤维酸性蛋白(glialfibrillaryacideicprotein,GFAP)的表达。方法:Wistar雄性大鼠20只随机分为MDMA组和对照组,MDMA组每天8:00,20:00两次给予MDMA(20mg/kg,腹腔注射×4d),对照组给予等体积的生理盐水。模型建成后,留取脑组织,分别采用高效液相色谱法测定不同脑区多巴胺、5-羟色胺含量,并采用免疫组织化学方法检测GFAP的表达。结果:MDMA可诱导实验大鼠的额叶皮质、海马和纹状体5-羟色胺的明显降低,分别为(9.26±1.44),(9.82±2.83),(31.66±0.11)ng/g,与对照组(29.06±1.18),(38.16±1.27),(50.56±0.70)ng/g比差异有非常显著性意义(t=30.436,20.394,32.693,P=0.000)。但MDMA对多巴胺影响不明显;上述3个脑区的GFAP表达明显上调。结论:MDMA对中枢5-羟色胺系统具有明显的神经毒性,可导致GFAP表达的上调。 AIM:To explore the neurotoxicity and glial fibriliary acidic protein(GFAP) expression of experimental rats induced by 3,4-methylenedioxy methamphetamine(MDMA). METHODS:Twenty male Wistar rats were randomly divided into MDMA group and control group.Rats in the MDMA group were injected intraperitoneally with MDMA(20 mg/kg) twice a day at 8:00 and 20:00 for 4 days,while those in the control group were injected with saline of the same volume.After the animal model was established,the brains were taken out, dopamine(DA) and 5-hydroxytryptamine(5-HT) in different brain areas were measured by high performance liquid chromatography(HPLC),and the expression of GFAP was detected by immunohistochemistry. RESULTS:MDMA resulted in the decrease of 5-HT in the frontal cortex, hippocampus and striatum, which were(9.26±1.44),( 9.82±2.83) and (31.66±0.11) ng/g respectively in the MDMA group,very significantly different from those in the control group[(29.06±1.18),(38.16±1.27), (50.56±0.70) ng/g](t=30.436,20.394,32.693,P=0.000),but MDMA had no significant influence on DA.The expressions of GFAP in the above-mentioned brain areas were obviously up-regulated. CONCLUSION:MDMA causes obvious neurotoxicity to the central 5-HT system,and up-regulates the expression of GFAP.
出处 《中国临床康复》 CSCD 2004年第31期6916-6917,i003,共3页 Chinese Journal of Clinical Rehabilitation
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