HCV核心区增强和抑制性多肽对小鼠CTL相互作用研究

Effects of inhibitory and enhancing polypeptides from HCV core region on cytotoxic T cell functions

目的 建立细胞毒性T细胞 (cytotoxicTcell ,CTL)检测体系 ,探讨丙型肝炎病毒 (hepatitisCvirus ,HCV)感染者体内CTL功能缺陷的原因。方法 选择HCV核心区多肽中对CTL有抑制作用和增强作用的多肽各 2条 ,交叉组合后共同皮下注射免疫BALB/c小鼠 ,用乳酸脱氢酶释放实验检测小鼠脾细胞CTL活性。结果 用LDH检测HCV核心区多肽免疫BALB/c小鼠的CTL活性 ,CTL活性可被CPA10 ( 5～ 2 3位氨基酸 )增强及被CPA9( 3 9～ 74位氨基酸 )抑制。CPB2 +CPB8、CPB6+CPB8组中效靶比 10∶1,2 0∶1的CTL活性显著高于对照组 ,CPB2 +CPB7、CPB6+CPB7组与对照组无明显差异 ,双因素方差分析显示HCV核心区抑制性多肽和增强性多肽有交互作用。结论 LDH可稳定检测BALB/c小鼠的CTL活性 ,HCV核心区抑制性和增强性多肽有相互作用。

Objective To investigate the pathogenesis of cytotoxic T cell (CTL) dysfunction in patients with HCV infection. Methods CTL detecting system was established. Two polypeptides which could enhance CTL function and two polypeptides which could inhibit CTL function were selected and cross-combined. BALB/c mice were immunized by subcutaneous injection of the combined polypeptides, and the CTL activity in mouse spleen cells was detected by LDH release test. Results CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV could be enhanced by CPA10 (5-23 aa) and inhibited by CPA9 (39-74 aa). CTL activity in the mice could be enhanced by polypeptides from the HCV core region, CPB2+CPB8, and CPB6+CPB8, respectively. There was no obvious difference between CPB2+CPB7, CPB6+CPB7 and the negative control. Two-factor analysis of variance showed that there was reciprocal action between the inhibitory and enhancing polypeptides from the HCV core region. Conclusion CTL activity in BLAB/c mice can be detected stably by LDH. There is an interactive effect between the inhibitory and enhancing polypeptides from the HCV core region.