前列腺按摩液中IL-8和TNF-α测定在慢性前列腺炎诊断、分型中的临床意义

IL-8 and TNF-α in Prostatic Secretions as Indications in Evaluating Chronic Prostatitis

目的 :探讨检测前列腺按摩液 (EPS)中细胞因子白细胞介素 8(IL 8)和肿瘤坏死因子α(TNF α)在慢性前列腺炎诊断、分型中的意义。 方法 :ELISA法检测 78例临床诊断的慢性前列腺炎患者 [其中慢性前列腺炎(CBP)组 12例 ,慢性非细菌性前列腺炎 /慢性骨盆疼痛综合征 (CPPS)ⅢA组 38例 ,CPPSⅢB组 2 8例 ]和 12例正常对照者EPS中IL 8和TNF α浓度。分析各组EPS中IL 8和TNF α浓度差异。 结果 :CBP组和CPPSⅢA组EPS中IL 8水平 [(10 96 7.5± 3477.7) pg/ml;(92 6 8.4± 2 0 34.6 ) pg/ml]和TNF α水平 [(84 .1± 5 4 .7) pg/ml;(32 .6± 18.6 ) pg/ml]显著高于CPPSⅢB组和正常对照组EPS中的IL 8水平 [(2 72 6 .1± 2 77.5 ) pg/ml;(2 80 0 .0± 32 0 .2 )pg/ml]和TNF α水平 [(12 .6± 7.1)pg/ml;(12 .9± 10 .1)pg/ml](P均 <0 .0 1)。 结论 :检测EPS中IL 8、TNF α水平可能有助于CBP、慢性非细菌性前列腺炎 /慢性骨盆疼痛综合征的分型诊断。

Objective: To investigate the significance of the determination of IL-8 and TNF-α in prostatic secretions in the evaluation of chronic prostatitis. Methods: IL-8 and TNF-α levels in EPS were evaluated by ELISA in 90 men: controls (n=12), CBP (n=12), CPPS ⅢA(n=38), CPPS ⅢB(n=28). And the difference was analyzed between CBP or CPPS ⅢA and CPPS ⅢB or controls. Results: IL-8 and TNF-α levels in EPS were higher in men with CBP \[( 10 967.5± 3 477.7) pg/ml, ( 84.1± 54.7) pg/ml\]or CPPS ⅢA \[( 9 268.4± 2 034.6) pg/ml and ( 32.6± 18.6) pg/ml\], but lower in men with CPPS ⅢB\[( 2 726.1± 277.5) pg/ml, ( 12.6± 7.1) pg/ml\] or controls \[( 2 800.0± 320.2) pg/ml and ( 12.9± 10.1) pg/ml\] respectively. There was significant difference between CBP or CPPS ⅢA and CPPS ⅢB or controls(P< 0.01). Conclusion: IL-8 and TNF-α are elevated in the EPS of the men with CBP and CPPS ⅢA, and provide a novel means for the identification and characterization of chronic nonbacterial prostatitis/chronic pelvic pain syndrome. The cut-points for IL-8 and TNF-α to discriminate CBP or CPPS ⅢA from CPPS ⅢB or controls need further investigation.