3种精子计数池的质量评估

Quality Evaluation of Three Different Sperm Counting Chambers

目的 :精液分析仍然是评估男性生育能力的一项重要的实验测试。然而 ,较少严格的医院男科学实验室精液分析质控使得比较不同实验室 ,甚至同一实验室在不同时间之间的精液分析结果变得非常困难或毫无意义。本文的目的是评价男科学实验室常用的血球计数池、Makler计数池及Cell VU计数池的准确性 ,为建立标准化的精液分析方法提供科学的实验依据。 方法 :以含已知浓度的 (HamiltonThorneBiosciences,USA)美国男科学实验室质控 (USandrologylabQC)乳胶珠溶液为QC标准溶液 ,分别用 3种计数池计数 ,并进行比较和分析。 结果 :Cell VU、血球计数池及Makler计数池计数的乳胶珠浓度分别为 (37.6 3± 4 .89)、(4 2 .74± 4 .98)、(5 3.5 2± 6 .6 7)×10 6/ml和 (18.2 2± 1.77)、(2 0 .4 8± 1.5 6 )、(2 4 .97± 4 .75 )× 10 6/ml,相应的两种标准溶液的浓度分别为 (35± 5 )和(18± 2 .5 )× 10 6/ml。Cell VU计数池的平均乳胶珠浓度类似于标准溶液 ,而血球计数池及Makler计数池的平均乳胶珠浓度显著高于Cell VU计数池 (P <0 .0 0 1)。与标准乳胶珠浓度相比 ,Cell VU计数池的平均变异系数 (CV)为7.5 1%和 1.2 2 % ,而血球计数池和Makler计数池的CV分别为 2 2 .11%、13.78%和 5 2 .91%、38.72 %。 结论 :不?

Objective: Semen evaluation is the most important laboratory test for assessing male fertility. However, lack of strict quality control (QC) for semen analyses in hospital andrology laboratories makes it difficult and meaningless to compare semen data between different laboratories. This paper reports a comparative study on the accuracy of the Hemacytometer (Qiujing Inc., Shanghai, China), Makler (Sefi-Medical Instrument, Haifa, Israel), and Cell-VU (Millennium Sciences Inc., New York, USA) chambers for sperm counting. Methods: Both low [(18± 2.5) ×10 6/ml] and high [(35±5)×10 6/ml] pre-calibrated standard latex bead solutions (Hamilton Thorne Biosciences,USA) were used as the quality control solution to perform counts on the three different counting chambers. Bead counts for the three different chambers were compared, and variability within the chambers determined for standard solutions at low and high concentrations of latex beads, respectively. Results: Mean bead concentrations for the Cell-VU, Hemacytometer and Makler chambers were ( 37.63± 4.89), ( 42.74± 4.98) and ( 53.52± 6.67)×10 6/ml respectively for a standard solution containing (35±5)×10 6 beads/ml , and ( 18.22± 1.77), ( 20.48± 1.56), ( 24.97± 4.75)×10 6/ml respectively for a standard solution containing (18± 2.5)×10 6 beads/ml. Mean bead concentrations for the Cell-VU chamber were consistently similar and close to the standard pre-calibrated bead solutions, while those for both the Hemacytometer and the Makler chambers were significantly overestimated (P< 0.001). The average coefficients of variation for the Cell-VU chamber were 7.51% for a higher concentration of the standard solution containing (36±5)×10 6 beads / ml and 1.22% for a lower concentration of the standard solution containing (18± 2.5)×10 6 beads/ml, while the mean variation rates of the Hemacytometer and Makler chambers were 22.11% and 13.78% for a standard solution containing (36±5)× 10 6 beads / ml, and 52.91% and 38.72% for a standard solution containing (18± 2.5)×10 6 beads/ml, respectively. Conclusion: Semen analysis is one of the most important tests for male fertility evaluation, but the data obtained from commercially available counting chambers may differ markedly in accuracy and reliability. Results from this comparative study demonsterated that the Cell-VU chamber exhibits significantly more accurate and less variable results than those of the Hemacytometer and Makler chambers. To ensure the best possible evaluations and accurate diagnoses, we therefore recommend that Cell-VU be used as the standard counting chamber for routine semen analyses in andrology laboratories.