哮喘患者PBMCs中NF-κB活性及血浆IL-4、IL-5水平关系的研究

Relationship between NF-κB activity in peripheral blood mononuclear cells and serum levels of interleukin-4 and interleukin-5 in patients with asthma

目的:探讨哮喘患者外周血单个核细胞(peripheralbloodmononuclearcells熏PBMCs)中核因子κB(NF鄄κB)活性及血浆IL鄄4、IL鄄5水平变化的关系。方法:用电泳迁移率分析法穴EMSA雪测定了22例发作期患者(哮喘组)、20例慢性阻塞性肺病患者(COPD组)及20例正常人(对照组)PBMCs中NF鄄κB活性,并用酶联免疫吸附法穴ELISA雪测定了血浆IL鄄4、IL鄄5水平。结果:哮喘组NF鄄κB活性(0.817±0.062)显著高于COPD组(0.757±0.051)、对照组(0.727±0.073)(P<0.01);哮喘组血浆IL鄄4(27.641±5.099)、IL鄄5(29.062±5.855)明显高于正常对照组IL鄄4(19.979±5.5710)、IL鄄5(20.476±5.517)(P<0.01);哮喘组NF鄄κB活性与血浆IL鄄4、IL鄄5水平呈正相关(r=0.599,r=0.654,P<0.01)。结论:通过抑制NF鄄κB活性下调IL鄄4和IL鄄5的表达,能在较高的水平上调控产生炎性因子的总体环节,更有效地控制哮喘。

Objective: To explore NF-κB activity in peripheral blood mononuclear cells(PBMCs)and serum levels of IL-4 and IL-5 and to study their relationship in patients with asthma. Methods: The NF-κB activity was measured by electrophoretic shift assay(EMSA)and the serum levels of IL-4 and IL-5 were measured by enzyme-linked immunoabsorbent assay(ELISA)in 22 patients with exacerbating asthma(asthma group), 20 with chronic obstructive pulmonary disease (COPD group) and 20 normal subjects (control group). Results: The NF-κB activity in asthma group (0.817±0.062)was significantly higher than those in COPD group (0.757±0.051)and control group(0.727±0.073)(P<0.01). The serum levels of IL-4(27.641±5.099)and IL-5 (29.062±5.855)in asthma group were also higher than those (19.979±5.571,20.476±5.517, respectively)in control group(P<0.01).The NF-κB activity had positive correlations with the serum levels of IL-4 and IL-5 (r=0.599 and 0.654, respectively) (P<0.01). Conclusion: The general steps of the production of inflammation factors can be controlled at a much higher level by inhibiting the NF-κB activity,and then downregulating the expressions of IL-4 and IL-5, so asthma can be treated more effectively by this mechanism.