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哺乳动物精子体外发生 被引量：4

In vitro spermatogenesis in mammalian
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摘要 精子的发生是一个高度复杂而有序的过程 ,涉及到细胞的增殖和分化。体外培养生精细胞在近年来取得了较大进展 ,建立了睾丸组织培养、曲精细管小段培养、支持细胞 生精细胞共培养及藻酸钙胶囊包裹培养等方法。建立生精细胞体外培养模型有助于 :(1)研究精子发生的调控机制 ;(2 )直接对雄性生殖细胞进行遗传修饰 ;(3)用于辅助生育技术 ,治疗精子发生阻滞的患者。如何改善培养条件 ,进一步提高生殖细胞的存活、分化、增殖效率 ,是使哺乳动物体外精子发生发展成为一项适用性较强的技术所必须解决的问题。
作者 孙燕 张家骅
机构地区 西南农业大学重庆市牧草与草食家畜重点实验室
出处 《生理科学进展》 CAS CSCD 北大核心 2004年第4期338-341,共4页 Progress in Physiological Sciences
基金 国家自然科学基金资助课题 (30 2 70 95 5 )
关键词 睾丸 生精细胞 体外精子发生
分类号 S814.8 [农业科学—畜牧学] Q954.4 [生物学—动物学]
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参考文献10

  • 1[1]Fujimoto K, Yamamoto T, Kitano T, et al. Promotion of cathepsin L activity in newt spermatogonial apoptosis induced by prolactin. FEBS Lett, 2002, 521:43～46.
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  • 3[3]Clifton RJ, O Donnell L, Robertson DM. Pachytene spermatocytes in co-cultre inhibit rat Sertoli cell synthesis of inhibin beta B-subunit and inhibin B but not the inhibin alpha-subunit. J Endocrinol, 2002, 172:565～574.
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同被引文献64

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  • 5Meng X, Pata I, Pedrono E, Popsueva A, de Rooij D G, Janne M, Rauvala H and Sariola H. Transient disruption of spermatogenesis by deregulated expression of neurturin in testis.Molecular of Cell Endocrinology, 2001,183: 33～39
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  • 7Soler R M, Dolcet X, Encinas M, Egea J, Bayascas J R and Comella J X. Receptors of the glial cell line-derived neurotrophic factor family of neurotrophic factor signal cell survival through the phosphatidylinositol 3-kinase pathway in spinal cord motoneurons. Journal of Neurology science,1999, 19(21):9160～9169
  • 8Tadokoro T Y, Yomogida K, Ohta H, Tohda A and Nishimune Y. Homeostatic regulation of germinal stem cell proliferation by GDNF/FSH pathway. Mechanism of Development, 2002,113(1):29～39
  • 9Viglietto G, Dolci S, Bruni P, Baldassarre G, Chiariotti L, Melillo R M, Salvatore G, Chiappetta G, Sferratore F, Fusco A and Santoro M. Glial cell line-derived neurotrophic factor and neurturin can act as paracrine growth factors stimulating DNA synthesis of Ret-expressing spermatogonia. Internationl Journal of Oncology, 2000, 16(4):689～94
  • 10Creemers L B, Meng X and den Ouden K. Transplantation of germ cells from Glial cell line-derived neurotrophic factor-overexpressing mice to host testes depleted of endogenous spermatogenesis by fractionated irradiation. Biology of Reproduction, 2002, 66:1579～1584

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生理科学进展
2004年 第4期

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