帕米磷酸钠对大鼠成骨细胞增殖、分化的影响

Effects of pamidronate on proliferation and differentiation of osteoblasts of rat

目的观察二磷酸盐类药物帕米磷酸钠对体外培养大鼠成骨细胞增殖、分化的影响。方法取新生SD大鼠头盖骨分离成骨细胞体外培养 ;不同浓度帕米磷酸钠刺激后 ,四甲基偶氮唑盐 (MTT)法检测细胞增殖能力 ;取细胞上清液 ,碱性磷酸酶试剂盒检测细胞培养液中碱性磷酸酶活性。结果在 10 6M— 10 12 M时 ,帕米磷酸钠能促进大鼠成骨细胞增殖 (P <0 .0 5 ) ,10 4M则抑制细胞增殖 (P <0 .0 5 ) ;帕米磷酸钠抑制大鼠成骨细胞碱性磷酸酶活性。结论帕米磷酸钠能直接作用于大鼠成骨细胞 ,促进其增殖 。

ObjectiveTo investigate effects of pamidronate on the proliferation and differentiation of osteoblasts of rats in vitro.MethodsOsteoblasts isolated from newborn rat calvaria were treated with various concentrations of pamidronate, the proliferation of osteoblasts was evaluated with the method of methyl thiazole tetrazolium (MTT) and activity of alkaline phosphatase (ALP) in medium was measured with kit of ALP detecting.ResultsThe proliferation of osteoblasts increased under the stimulation of Pamidronate range 10 6 M-10 12 M ( P<0.05), but was inhibited at the concentration of high level (10 4 M). The activity of ALP decreased in the experiment.ConclusionPamidronate can act on the osteoblasts directly and increase the proliferation of bone cells, but inhibit the differentiation of the same cells.