人参皂苷Rg1和Rh1对树突状细胞功能的影响(英文)

Effect of ginsenoside Rg1 and Rh1 on the anti-tumor activity of dendritic cell

目的 :研究人参皂苷Rg1和Rh1对树突状细胞 (dendriticcell,DC)功能的影响。方法 :用ELISA法检测人参皂苷Rg1及Rh1对DC的IL - 12p4 0蛋白产生量的影响 ,用RT -PCR检测人参皂苷Rg1及Rh1对DCIL - 12p4 0mRNA表达水平的影响。用中性红吞噬法检测人参皂苷Rg1及Rh1对DC -LPAK对肿瘤细胞的杀伤作用。结果 :ELISA结果表明 ,Rg1和Rh1各剂量组均能显著提高DCIL - 12p4 0蛋白的产量。与对照组相比 ,Rg11mg/L及Rh110 0mg/L可明显增加DCIL - 12p4 0mRNA的转录 ,与其蛋白表达相一致。Rg1及Rh1均能促进DC -LPAK对乳头瘤细胞的杀伤能力 ;在对L92 9杀伤实验中 ,效靶比为 5∶1时 ,Rg1各剂量均能显著促进DC -LPAK的杀伤活性(P <0 0 1,P <0 0 5 ) ,而Rh1仅中剂量能提高DC -LPAK的杀伤活力 (P <0 0 5 )。结论 :Rg1和Rh1通过增强IL - 12p4 0mRNA的表达来增加其蛋白的产量。并且 ,由于IL - 12产量的增加从而增强了DC

AIM: To study the effect of ginsenoside Rg1 and Rh1 on the anti-tumor activity of dendritic cells (DC). METHODS: Effect of Rg1 and Rh1 on the production of IL-12 p40 protein was detected by ELISA, and the IL-12 p40 mRNA level of DC was monitored by RT-PCR. Anti-tumor activity of DC-LPAK was determined by neutral red staining assay. RESULTS: The results of ELISA showed that Rg1 and Rh1 significantly enhanced the production of IL-12 p40 of DC. Rg1 at 1 mg/L and Rh1 at 100 (mg/L) upregulated the IL-12 p40 mRNA level. Rg1 and Rh1 enhanced the anti-tumor ability of DC, induced lymphokine and PHA activated killer (DC- LPAK) on human papillate tumor cell line. Each dose of Rg1 can obviously accelerate the cytotoxity to L929 at the E∶T ratio of 5∶1(P<0.05,0.01), while only Rh1 10 (mg/L) enhanced the cytotoxity ability of DC-LPAK (P<0.05). CONCLUSION: Rg1 and Rh1 enhanced the production of IL-12 p40. This effect may be mediated by the increase in the mRNA level. As a result, Rg1 and Rh1 promote the ability of DC to stimulate the cytotoxitic acticity of DC-LPAK. [