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CpG ODN对树突状细胞抗肝癌作用的影响 被引量：2

Effects of CpG ODN on dendritic cells in anti-hepatocarcinoma action
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摘要 目的 :研究CpGODN在BALB/C小鼠骨髓来源树突状细胞 (BMDC)抗肝癌免疫中的作用。方法 :CpGODN联合肿瘤抗原 (TAg)体外刺激BMDC ,流式细胞术检测受刺激BMDC表达CD80的阳性率 ,ELISA检测受刺激BMDC分泌IL - 12 (p70 )的水平 ,MTT法检测活化BMDC刺激T细胞的增殖能力 ,联合CpGODN和TAg体内预激小鼠获得CTL ,检测CTL对肝癌细胞株Hca-F的特异性杀伤作用。结果 :CpGODN联合TAg体外可明显激活BMDC ,诱导BMDC膜表面CD80的表达 ,刺激BMDC分泌高水平IL - 12 ,促进BMDC刺激T淋巴细胞增殖 ;体内联合应用CpGODN和TAg诱导的CTL对Hca -F具有强大的特异性杀伤作用 ,其诱导作用明显强于TAg ,与细菌脂多糖和金黄色葡萄球菌肠毒素B相近。结论 :CpGODN体外能有效促进小鼠BMDC的分化、增殖和成熟 。 AIM: To study the effect of oligonucleotides containing unmethylated CpG motif (CpG ODN) on mouse bone marrow-derived dendritic cells (BMDCs) in anti-HcaF cytotoxicity. METHODS: BMDCs were stimulated by CpG ODN in combination with tumor antigen (TAg). The expression of CD80 on BMDC surface was analyzed by FCAS. Level of IL-12 (p70) in supernatants of BMDC culture was detected by ELISA. The proliferation of T cells was examined by MTT assay. Cytotoxicity of CTL induced by CpG ODN combining with TAg was detected by MTT assay. RESULTS: CpG ODN combining or not combining with TAg up-regulated the expression of CD80 on BMDC surface and stimulated BMDCs to produce a high level of IL-12. CpG ODN-activated BMDC promoted the proliferation of T cells. CTL induced by CpG ODN in combination with TAg appeared strong specific cytotoxicity on Hca-F cells. CONCLUSION: CpG ODN may effectively induce the functional maturation of mouse BMDC in vitro. CpG ODN in combination with TAg can enhance the anti-HcaF cytotoxicity of CTL. [
作者 李岩 邢飞跃 李明
机构地区 四川大学华西医学中心感染免疫研究室 暨南大学教育部组织移植与免疫重点实验室
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2004年第10期1801-1805,共5页 Chinese Journal of Pathophysiology
关键词 胞嘧啶鸟嘌呤二核苷酸 寡二核苷类 树突状细胞 T淋巴细胞 细胞毒性 Cytosine-phosphate-guanine Oligonucleotides Dendritic cells T-lymphocytes, cytotoxic
分类号 R730.5 [医药卫生—肿瘤]
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参考文献13

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共引文献13

同被引文献17

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中国病理生理杂志
2004年 第10期

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