白藜三醇对氧自由基致兔血管平滑肌细胞NF-κB活化及MCP-1表达的影响

Intervention of resveatrol on activated nuclear factor-κB and expression of monocyte chemotactic protein-1 in cultured rabbit aortic smooth muscle cells induced by xanthine and xanthine oxidase

目的 :探讨白藜三醇 (RES)对X/XO致血管平滑肌细胞内核转录因子NF -κB活性和单核细胞趋化蛋白 - 1表达 (MCP - 1)的影响。方法 :以培养幼兔主动脉平滑肌细胞为研究对象 ,分别给予不同剂量的X/XO和 /或RES ,采用MTT法、电泳迁移率改变分析法、免疫组织化学和原位杂交技术检测不同处理组平滑肌细胞增殖及NF -κB活性和MCP - 1蛋白及其mRNA表达的变化。结果 :不同浓度的X/XO所产生的氧自由基可明显增加体外培养SMC增殖及NF -κB的活性和MCP - 1蛋白及其mRNA的表达水平 ,RES呈剂量依赖性地抑制氧自由基对体外培养SMC的增殖作用和NF -κB的活性 ,并下调MCP - 1的表达水平 ;其中以终浓度 10 0 μmol/L的RES对氧自由基介导的NF -κB活性的抑制作用最强 ;终浓度 2 0 0 μmol/L的RES对SMC的增殖作用及MCP - 1表达的抑制作用最强。 结论 :RES具有抑制X/XO产生氧自由基对NF -κB的诱导合成和MCP -

AIM: To investigate the role of resveatrol (RES) among red wine on the proliferation, activity of NF-κB and the expression of monocyte chemotactic protein-1 (MCP-1) induced by xanthine and xanthine oxidase in cultured rabbit aortic smooth muscle cells (SMC). METHODS: SMC proliferation was examined by 3-4, 5-dimethylthiazol-2-yl-2, 5-diphenylte tra zoliumbromide (MTT) metabolism, activity of NF-κB, the protein and mRNA expression of MCP-1 were detected by electrophoretic mobility shift assay (EMSA), immunohistochemitry and in situ hybridyzation in cultured rabbit aortic SMC. RESULTS: 100 μmol/L-200 μmol/L resveatrol, an effective composition in red wine, was confirmed to inhibit MTT metabolism and the activity of NF-κB as well as the protein and mRNA expression of MCP-1 in rabbit aortic SMC, which were promoted by the oxygen free radicals induced by xanthine and xanthine oxidase. CONCLUSION: Resveatrol may antagonist oxygen free radicals-induced proliferation and the activity of NF-κB as well as protein and mRNA expression of MCP-1 in cultured rabbit aortic SMC, which might play an important role in preventing atherosclerosis. [