创伤应激对正常和糖尿病大鼠脾淋巴细胞免疫功能的影响

Affection of Immune Function on Spleen Lymphocyte of Rat with Traumatic Stress for Normai and Diabetes

目的:观察创伤应激对正常和糖尿病大鼠脾淋巴细胞免疫功能的影响.方法:采用MTT法、ELISA法分别观察正常大鼠和糖尿病大鼠创伤应激前后脾淋巴细胞增殖反应和ConA诱生的脾淋巴细胞TNF-α、IL-6分泌水平.结果:与正常组大鼠比较,糖尿病大鼠脾淋巴细胞增殖反应被里著抑制,ConA诱生的脾淋巴细胞TNF-α、IL-6分泌水平增高;创伤应激后,正常及糖尿病大鼠脾淋巴细胞增殖反应均被显著抑制,ConA诱生的脾淋巴细胞TNF-α、IL-6分泌水平增高.结论:创伤应激可损伤正常大鼠的脾淋巴细胞免疫功能,并使糖尿病大鼠已低下的细胞免疫功能进一步受损.

Objective: To observe the immune functional affection of rat spleen lymphocyte with traumatic stress for normai and diabetes. Methods: The spleen lymphocyte proliferation, TNF-a induced by ConA, and Ievel of IL-6 were observed for normai and diabetes rats with MTT and EL1SA methods. Results: Compared with normai rat, the proliferation of diabetes rat was inhibited significantly and the ConA induced TNF-a, IL-6 Ievel increased. After traumatic stress, splenic lymphocyte proliferations were inhibited in all groups and the ConA induced TNF-a, IL-6 Ievel increased. Conclusion: The traumatic stress could injury the spleen lymphocyte immune function of normai rat and could further damage the diabetes rat's function which was low Ievel.