生存素mRNA、生存素和PCNA在大肠癌中的表达及意义

Expression and Significance of Survivin and PCNA in Human Colorectal Carcinoma

目的 探讨SurvivinmRNA、Survivin和PCNA在大肠癌中的表达及意义。方法 原位杂交、免疫组化法检测 64例大肠癌中SurvivinmRNA、Survivin、PCNA蛋白表达。结果 SurvivinmRNA、Survivin蛋白在大肠癌的阳性率分别为 5 3 %、5 3 % ,其表达与大肠癌组织类型、淋巴结转移相关 (P <0 0 5 ) ,而与侵犯深度无关 (P >0 0 5 )。癌旁组织中无Survivin表达。PCNA增殖指数为 ( 3 3 65± 18 7) %。Survivin与PCNA蛋白表达呈正相关。结论 大肠癌中Survivin高表达 ,其与大肠癌分化程度、淋巴结转移、增殖活性有关。Survivin基因有望成为大肠癌诊断、治疗的新靶点。

Objective To evaluate the expression and significance of survivin and PCNA in human colorectal carcinoma. Methods Using in situ hybridization and immunohistochemistry(S-P method) respectively to detect survivin mRNA, and survivin and PCNA proteins in 64 cases of colorectal carcinoma. Results The survivin expression wasn't detected in the tumor adjancent tissues. The positive rate of survivin mRNA and protein expressions was 53% and 53% respectively, which was significantly associated with the histological type and lymph node metastasis of colorectal carcinoma (P<0.05). PCNA labelling index in the colorectal carcinoma was 33.65±18.7%. There was a positive correlation between survivin and PCNA protein expressions in the colorectal carcinomas. Conclusion Survivin expression increased in colorectal carcinoma,which was related to histological type,lymph node metastasis and proliferation potential of colorectal carcinomas. Survivin may be a new diagnostic/therapeutic target of human colorectal carcinoma.