乌司他丁对急性肺损伤兔炎性细胞因子及氧自由基的影响

The Effects of Ulinastatin on Proinflammatory Cytokine and Oxygen Free Radicals During Acute Lung Injury in Rabbits

目的 研究乌司他丁在兔急性肺损伤时对炎性细胞因子及氧自由基代谢的影响。方法 将 18只健康新西兰大白兔随机分为 2组。油酸组 (n =9)静脉注射分析纯油酸 0 12ml/kg ,复制急性肺损伤模型。乌司他丁组 (n =9)静脉注射乌司他丁5 0 0 0 0u/kg后立即静脉注射油酸 0 12ml/kg ,然后 ,再予乌司他丁 5 0 0 0u·kg-1·h-1持续静脉输注。两组皆在操作完成稳定 10min后 (基础值 )及注射油酸完毕后 1h、2h、3h、4h抽静脉血测血浆IL -8、MDA和活性氧含量 ,抽取动脉血作血气分析。结果 两组的IL -8、MDA和活性氧含量以及PaCO2 在注射油酸后逐渐上升 ,PaO2 逐渐下降 ,油酸组各观察指标的值的变化大于乌司他丁组 ,差异具有显著性。结论 乌司他丁能减少急性肺损伤中的IL -8及氧自由基的生成和释放。

Objective To study the effects of ulinastatin on proinflammatory cytokine and oxygen free radicals during acute lung injury in rabbits. Methods Eighteen health New Zealand rabbits were randomly divided into 2 groups: oleic acid group(n=9) and ulinastatin group(n=9). The rabbits of oleic acid group were intravenously injected analytical pure oleic acid(0.12ml/kg) to set up a model of acute lung injury. The rabbits of ulinastatin group were intravenously injected ulinastatin 50000u/kg before injecting oleic acid and received intravenous infusion of ulinastatin 5000u·kg -1 ·h -1 after injecting oleic acid. Before injecting oleic acid and 1h, 2h, 3h and 4h after injecting oleic acid,venous blood samples were taken to determine the concentration of plasma IL-8, MDA and active oxygen,and arterial blood samples were also taken for gas analysis. Results The concentration of IL-8, MDA and active oxygen and PaCO 2 gradually increased,and PaO 2 gradually decreased after injecting oleic acid in the both groups of rabbits. The changes of the above indices in the oleic acid group were larger than those in the ulinastatin group, and the difference between the two groups was significant. Conclusion Ulinastatin inhibited the release of proinflammatory cytokine and reduced the production of oxygen free radicals during acute lung injury.