2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖诱导肝癌细胞凋亡的形态学变化

Morphology of apoptosis of human hepatocellular carcinoma cell line induced by 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose

目的:探讨2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖诱导肝癌细胞株HepG2凋亡的可能性. 方法:选用不同浓度的2-(3-羧基-1-丙酰氨基)-2-脱氧- D-葡萄糖与肝癌细胞株共同孵育不同时间后,应用倒置相差显微镜、荧光显微镜、透射电子显微镜对细胞生长状况及药物作用后细胞的形态进行观察. 结果:经2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖作用24-72 h后,HepG2肝癌细胞株出现体积缩小,荧光染色增强,胞核或胞质中可见致密浓染的块状或颗粒状黄绿色荧光染色.染色质固缩,并凝结成块,聚集在核膜周边呈新月状或肾状,胞质浓缩,内质网疏松并与胞膜融合形成一个个空泡.且凋亡细胞含量呈一定的浓度、时间相关性. 结论:2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖具有诱导HepG2细胞凋亡的作用.

AIM: To investigate the changes of apoptosis in hepato-cellular carcinoma cells induced by 2-[(3-carboxy-l-oxoprogy1) amino]-2-deoxy-D-glucose. METHODS: Fluorescent microscopy and transmission electron microscopy were used to observe the morphologic changes of apoptosis with different concentration of 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose at different time. RESULTS: The morphologic changes of apoptosis were exhibited, including shrinkage of cell, condensation of chromatin, breakage of nuclear and formation of apoptotic bodies, but the number of apoptosis increased with the concentration 2-[(3-carboxy-1-oxoprogy1) amino]-2-deoxy-D-glucose and time. CONCLUSION: 2-[(3-carboxy-1-oxoprogyl) amino]-2-deoxy-D-glucose can induce apoptosis of human hepatocelluar carcinoma cells (HepG2).