肝纤维化发生发展过程中肝温度的变化规律

Alterations of liver temperature during hepatic fibrosis

目的:研究肝纤维化发生、发展过程中肝温度变化的规律方法:采用医用红外热像技术检测造模2,4,6,8wkCCl4 肝纤维化大鼠模型及对照组大鼠肝脏温度,肝组织芯片行苏木素-伊红、天狼猩红及免疫组织化学染色,观察肝组织病理变化,Ⅰ,Ⅲ型胶原及层粘蛋白的表达. 结果:正常大鼠肝右叶2,4,6,8 wk平均温度为28.1±1.0℃,27.2±0.8℃,30.7±1.0℃,29.1±0.7℃.肝纤维化大鼠模型组2 wk肝平均温度较对照组明显升高(P.<0.05, t=-2.518);模型组4 wk与对照组肝无明显差异,模型组6,8 wk肝平均温度较对照组明显降低(P<0.05 t=3.773, P<0.01 t=4.998);模型组2 wk病理变化以炎症及肝细胞变性为主,模型组4 wk间质成分增生明显,纤维化逐渐形成,模型组6 wk肝细胞大量坏死,中央静脉及汇管区纤维隔形成,模型组8 wk肝组织形成完整的假小叶;模型组2,4,6,8 wk大鼠肝组织Ⅰ,Ⅲ型胶原及层粘蛋白表达逐渐增多,2,4 wk以Ⅲ型胶原增加为主,4,8 wk 以Ⅰ型胶原增加为主. 结论:肝纤维化发生、发展的不同阶段,肝温度呈规律性变化.

AIM: To investigate the alterations of liver temperature during hepatic fibrosis in rats induced by CCl4 METHODS: Liver fibrosis was induced in rats after administration of CCl4 twice a week for 8 weeks. The rat liver temperature in 2-, 4-, 6- and 8- wk models and control group rat was detected by computer-assisted infrared thermorgraphic technology. To observe the histological change, the liver tissue microarray in every group of animals was stained by hematoxylin and eosin. Sirius red stain and polarization microscopy method was used for I and Ⅲ collagen expression. Immunohistochemistry was used for detection of laminin to outline the formation of basement membrane on hepatic sinusoids. RESULTS: The average infrared images temperature of hepatic fibrosis in rats induced by CCl4 changed with the time of the model made. In 2-wk model group, the infrared image temperature of right liver lobe was 28.6±1.1℃, compared with 2-wk control group (P<0.05, t =-2.518). The temperature of 4-wk model group was 27.2±1.0℃. In 6-wk model group, the temperature was 30.1 ±1.2℃, compared with 6 wk control group (P<0.05, t=3.773). The temperature of 8-wk model group was 27.4±1.0℃, compared with 8 wk control group (P<0.01, t =4.998). The histological changes of liver tissue were inflammation and hepatocytic degeneration and necrosis in 2-wk model group. There was a significant increase of extracellular matrix among the necrostic hepatocytes in 4-wk model group. In 6-wk model group liver tissue showed the numbers of necrostic hepatocytes increased significantly and the septa formed around central vein and portal area. In 8 wk model group, there were wide septa expansions from the portal area to the central venous and pseudo-nodular formation. Sirius red and immunohistochemical stains showed collagen types Ⅰ, Ⅲ and laminin expression in liver tissues increased gradually in 2-, 4-, 6-, 8- wk model group. Collagen type Ⅰ was the main collagen in 2- and 4-wk model groups. Collagen type I was the main collagen in 6-and 8-wk model groups. CONCLUSION: The liver temperature changes regularly with hepatic tissue pathology in course of rat hepatic fi-brosis induced by CCl4.