期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

三叶因子1在胃黏膜中的表达及存在的分子形式 被引量：1

下载PDF
导出
摘要 目的:研究三叶因子1在人胃黏膜中的表达及存在的分子形式,探讨其胃黏膜保护作用的分子生物学机制. 方法:采用western-blot方法观察三叶因子1在人胃黏膜中的存在形式,并采用免疫组化方法了解三叶因子1在胃黏膜中的定位,比较其在胃溃疡周边及距周边5cm外胃黏膜中的表达改变. 结果:(1)三叶因子1在人胃黏膜中以三种形式存在:单聚体、二聚体及一种分子量约为21kDa的TFF1与某种分子结合而成的复合物,其中,TFF1复合物的含量最高.(2) 三叶因子1主要在人胃体及胃窦黏膜上皮表面细胞胞质中表达,核周聚集较明显,越靠近腔面的细胞表达越强.(3) 三叶因子1在胃溃疡周边黏膜的表达明显高于距溃疡周边5cm外的黏膜(P<0.001). 结论:三叶因子1主要在人胃体及胃窦黏膜上皮表面细胞胞质中表达,其与某种分子结合形成的复合物是TFF1在胃黏膜中存在的主要形式,这种TFF1复合物可能比TFF1单聚体及二聚体有更强的生物活性.TFF1在胃溃疡周边黏膜表达高于距溃疡周边5cm外的黏膜,说明其在胃黏膜保护及修护方面具有重要的作用.
作者 任建林 罗金燕 卢雅丕 王琳 陈建民 刘明 施华秀
机构地区 西安交通大学第二医院消化内科 厦门大学医学院第一临床学院
出处 《世界华人消化杂志》 CAS 2004年第9期2186-2188,共3页 World Chinese Journal of Digestology
关键词 三叶因子 胃黏膜 表达 胃溃疡 分子形式 胃体 胃窦黏膜 方法观 结论 定位
分类号 R573 [医药卫生—消化系统] R57 [医药卫生—消化系统]
  • 相关文献

参考文献17

  • 1Masiakowski P, Breathnach R, Bloch J, Gannon F, Krust A,Chambon P. Cloning of cDNA sequences of hormone regulated genes from the MCF-7 human breast cancer cell line. Nucleil Acids Res 1982;10:7895-7903.
  • 2Chadwick MP, Westley BR, May FE. Homodimerization and hetero-oligomerization of the single-domain trefoil protein pNR-2/pS2 through cysteine 58. Biochem J 1997;327(Pt 1):117-123.
  • 3Ribieras S, Tomasetto C, Rio MC. The pS2/TFF1 trefoil factor,from basic research to clinical applications. Biochimica et Biophysica Acta 1998; 1378:F61-77.
  • 4Tanaka S, Podolsky DK, Engel E, Guth PH, Kaunitz JD. Human spasmolytic polypeptide decreases proton permeation through gastric mucus in vivo and in vitro. Gastrointest Liver Physiol 1997;35:G1473-1480.
  • 5Thim L, Mortz E. Isolation and characterization of putative trefoil peptide receptors. Regulatory Peptides 2000;90:61-68.
  • 6Tan XD, Hsueh W,-Chang I-I, Wei KR,Gonzalez-Crussi F.Characterization of a putative receptor for intestinal trefoil factor in rat small intestine: identification by in situ binding and ligand blotting. Biochem Biophys Res Commun 1997; 237:673-677.
  • 7Newton JL, Allen A, Westley BR, May FEo The human trefoil peptide, TFF1, is present in different molecular forms that are intimately associated with mucus in normal stomach. Gut 2000;46:312-320.
  • 8王蔚,口如琴,李令媛,茹炳根.三叶肽:从结构到功能[J].生命科学,2001,13(3):100-102. 被引量：8
  • 9Pera M,Heppell J, Poulsom R, Teixeira FV, Williams J. Ulcer associated cell lineage glands expressing trefoil peptide genes are induced by chronic ulceration in ileal pouch mucosa. Gut 2001:48:792-796.
  • 10任建林,卢雅丕,王琳,陈建民,施华秀,叶震世,吴艳环,钟燕,林逊汀,林辉,潘金水,罗金燕.TFF1在正常及损伤胃黏膜中的表达改变[J].世界华人消化杂志,2003,11(11):1809-1810. 被引量：13

二级参考文献32

  • 1[1]Thim L. Digestion, 1994; 55(2): 353-356.
  • 2[2]Seib T, Blin N, Hilgert K, et al. Genomics, 1997;40(1): 200-202.
  • 3[3]Cart M D, Bauer C J, Gradwell M J, et al. Proc Natl Acad Sci USA, 1994; 91(6): 2206-2210.
  • 4[4]Amitabha D E, Brown D G, Gorman M A, et al. Proc Natl Acad Sci USA, 1994; 91(3): 1084-1088.
  • 5[5]Richard P, Denis E B, Irivin M M. Yale J Biol Med,1997; 69(2): 137-146.
  • 6[6]Sarraf C E, Alison M R, Ansari T W, et al. Microsc Res Tech, 1995; 31(3): 234-237.
  • 7[7]Taupin D, Ooi K, Yeomans N, et al. Lab Invest, 1996;75(1): 25-32.
  • 8[8]Rio M C, Chenard M P, Wolf C, et al. Gastroenterology, 1991; 100(2): 375-379.
  • 9[9]Babyatsky M W, Debeaumont M, Thim L, et al. Gastroenterology, 1996; 110: 489-497.
  • 10[10]Wright N A, Pike C, Elia G. Nature, 1990; 343(1):82-85.

共引文献24

同被引文献28

  • 1Machado J.C,Nogueira A.M.,Carneiro F,et al.Gastric carcinoma exhibits distinct types of cell differentiation:an immunohistochemical study of trefoil peptides (TFF1 and TFF2) and mucins (MUC1,MUC2,MUC5AC and MUC6)[J].J Pathol,2000;190(4):437-443
  • 2Leung WK,Yu J,Chan FK,et al.Expression of trefoil peptides (TFF1,TFF2 and TFF3) in gastric carcinomas,intestinal metaplasia,and non-neoplastic gastric tissues[J].J Pathol,2002; 197(5):582-588
  • 3Lefebvre O,Chenard M.P,Masson R,et al.Gastric mucosa abnormalities and tumorigenesis in mice lacking the pS2 trefoil protein[J].Science,1996;274(5285):259-262
  • 4Tomasetto C and Rio MC.Pleiotropic effects of trefoil factorl deficiency[J].Cell Mol Life Sci,2005;62(24):2916-2920
  • 5Emami S,Le Floch N,Bruyneel E,et al.Induction of scattering and cellular invasion by trefoil peptides in src-and RhoA-transformed kidney and colonic epithelial cells[J].FASEB J,2001; 15(2):351 -361
  • 6Bossenmeyer-Pourie C,Kannan R,Ribieras S,et al.The trefoil factor 1 participates in gastrointestinal cell differentiation by delaying G1S phase transition and reducing apoptosis[J].The Journal of Cell Biology,2002;157(5):761-770
  • 7Rodrigues S,Nguyen QD,Faivre S,et al.Activation of cellular invasion by trefoil peptides and src is mediated by cyclooxygenase and thromboxane A2 receptor-dependent signaling pathways[J].FASEB J,2001; 15(9):1517-1528
  • 8Emami S,Rodrigues S,Rodrigue CM,et al.Trefoil factor family (TFF) peptides and cancer progression[J].Peptides,2004;25(5):885-898
  • 9Rodrigues S,Attoub S,Ngnyen QD,et al.Selective abrogation of the proinvasive activity of the trefoil peptides pS2 and spasmolytic polypeptide by disruption of the EGF receptor signaling pathways in kidney and colonic cancer cells[J].Oncogene,2003;22(29):4488-4497
  • 10Pai R,Soreghan B,Szabo IL,et al.Prostaglandin E2 transactivates EGF receptor:a novel mechanism for promoting colon cancer growth and gastrointestinal hypertrophy[J].Nat Med,2002; 8 (3):289-293

引证文献1

二级引证文献1

世界华人消化杂志
2004年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部