唾液腺腺样囊性癌p53基因突变及其与临床病理的关系

Analysis of p53 gene mutation in salivary adenoid cystic carcinoma

目的:研究唾液腺腺样囊性癌(ACC)p53基因突变及其与临床病理因素的关系。方法:选择38例唾液腺ACC病理标本,采用聚合酶链式反应单链构象多态性分析(PCR-SSCP)进行p53基因突变(5-8外显子)的检测;应用SPSS10.0统计学分析软件,采用Kaplan-Meier法进行生存分析,并绘制生存曲线。p53基因突变与ACC各病理类型、局部复发率、远处转移率及临床分期的关系采用Fisher精确检验。结果:p53基因5-8外显子突变检出率为65.8%,p53基因突变与唾液腺ACC的肿瘤复发、远处转移及术后生存有关(P<0.05),与肿瘤病理类型、临床分期无关(P>0.05)。结论:p53基因突变在唾液腺ACC中有较高的检出率,并可能与患者的预后有关。

PURPOSE: The purpose of this study was to investigate the occurrence of p53 gene mutation in salivary adenoid cystic carcinoma, the correlation between the gene and prognostic significance. METHODS: A series of salivary adenoid cystic carcinoma were studied by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) analysis to detect p53 mutation, respectively. RESULTS: The result showed that p53 gene mutations in exon 5-8 were detected in 65.8% (25/38) of the salivary adenoid cystic carcinoma. The p53 gene mutation was related to tumor distant metastasis and tumor recurrence (P<0.05) but not related to pathological types, clinical stages (P>0.05).There was a significant correlation between p53 gene mutation and postoperative survival . CONCLUSION: It is suggested that p53 gene mutations were very common in salivary adenoid cystic carcinoma, and might be useful in screening progress and evaluating prognosis of patients.