参附注射液对肠缺血-再灌注大鼠肿瘤坏死因子α的影响

Effect of shen-fu injection on tumor necrosis factor after intestinal ischemia reperfusion in rats

目的观察肿瘤坏死因子α(TNF-α)在大鼠肠缺血-再灌注损伤过程中的作用及参附注射液对TNF-α的影响,探讨参附注射液防治肠缺血-再灌注损伤机制。方法 SD大鼠随机分为肠缺血-再灌注组(IR组)、参附注射液预处理组(SF组)和假手术组(C组)。采用阻断肠系膜上动脉(SMA)的方法制造肠缺血-再灌注模型。分别测定各组动物血浆、肠组织TNF-α含量及血液动力学变化;光镜观察肠粘膜损伤情况。结果IR组再灌注后MAP下降,与C组和SF组比有显著性差异(P<0.01);SF组肠粘膜损伤程度减轻,与IR组比有显著性差异(P<0.01);SF组血浆及肠组织TNF-α水平降低,与IR组比有显著性差异(P<0.01)。结论参附注射液可明显防治大鼠肠缺血-再灌注导致的肠粘膜损伤,这种作用可能是通过抑制TNF-α的释放实现的。

Objective To investigate the potential role of tumor necrosis factor-α(TNF-α) in intestinal ischemia-reperfusion injury,and the effect of shen-fu injection on TNF-a release after intestinal ischemia-reperfusion. Methods Adult Sprague-Dawiey rats were subjected to intestinal ischemia (1 hour of superior artery occlusion alone) followed by 2 hours of reperfusion. Animals were divided into three groups: (1)ischemia and reperfusion alone(n=12) , (2) shen-fu injection pretreatment (10 ml/kg,30 min before ischemia,n -12) ,(3) sham operated controls (laparotomy,n = 12). The expression levels of TNF-α in the small-intestinal tissue and plasma were evaluated by enzyme-linked immu-noadsorption assay (ELISA)in each group. Results Pretreatment with shen-fu injection improved the hypotensive status caused by intestinal ischemia-reperfusion ( P< 0. 01), alleviated the pathologic lesion of small intestine mucosa (P<0. 01), decreased the elevation of plasma and small-intestinal tissue TNF-α levels after intestinal ischemia-reperfusion (P<0. 01). Conclusion Pretreatment with shen-fu injection can prevent intestinal ischemia-reperfusion injury in rats,and such protective effect may be related to the inhibition of TNF-α release.