紫外分光光度法测定罗红霉素胶囊的含量

Determination of roxithromycin in roxithromycin capsules by UV spectrophotometry

目的采用紫外分光光度法测定罗红霉素胶囊的含量。方法利用罗红霉素在 4 82nm波长处有最大吸收 ,而空白辅料在此处无吸收。结果罗红霉素在 16 .6 6～ 5 0 .0 0 μg/mL浓度范围内 ,吸收度与浓度之间呈良好的线性关系 (r=0 .9999) ;样品的平均回收率为 10 0 .2 % ,RSD =0 .5 % (n =9)。结论该方法操作简便 ,结果准确可靠 。

Objective To develop a UV method for the determination of roxithromycin. Methods roxithromycin was determined at 482 nm and blank control had little interference. Results The calibration curve was linear in the range of 16.66～50.00 μg/mL for roxithromycin and the correlation coefficient was 0.9999, The average recovery was 100.2%, with RSD of 0.5% (n=9). Conclusion The method is simple, accurate and was suitable for the determination of roxithromycin preparations.