抗加特纳杆菌卵黄免疫球蛋白(IgY)的研究

Studies of Immunoglobulin of yolk (IgY) against Gardnerella

目的观察鸡卵黄中抗加特纳杆菌抗体的产量、纯度、来源及稳定性。方法用阴道加特纳杆菌作为抗原免疫Leghorn鸡。通过改良水稀释法提取卵黄中的IgY。双紫外光波长测定抗体含量 ,SDS -PAGE电泳检测抗体纯度。Westernblot免疫印迹法测定该抗体来源。ELISA检测IgY对温度、酸碱度的稳定性。结果抗体含量 12 .8mg/mL蛋黄液 ,抗体纯度达 95 %。免疫印迹证明IgY与鸡血清中的IgG具有相同的分子量和抗原性。IgY具有良好的热稳定性 ,对酸碱具有一定的耐受力。结论WD水稀释法能得到高产量、高纯度的特异性IgY 。

Objective to observe the yield, purity and stability of IgY against Gardnerella. Methods laying hens (White Leghorn) were immunized with G. Vaginalis. IgY was purified with the modified simple water dilution method (WD) and its purity and yield were measured respectively with SDS-PAGE and ultraviolet spectrophotometry. The antigenicity of IgY anainst heating, acid and alkali, repeated freezing and fusion treatments were researched with enzyme-linked immunoadsorbent assay (ELISA) technology. Results purity of IgY was about 95% and 12.8 mg IgY from per milliliter egg yolk was routinely obtained. Moreover, IgY had the same molecular weight and antigenicity in comparison with IgG of chick serum. The IgY was fairly heat-stable and had a certain degree of acid-alkali resistance. Conclusion high yield and purity of specific antibody IgY from egg yolk was obtained successfully by WD purification methods, and it showed good biological activities.