抗人尿道高分化鳞癌单抗E_6及F_9在上皮性肿瘤组织的表达及其意义

The expression and significance of E_6 and F_9 high in epithelioid tumour tissue

目的 探讨E6,F9在鳞癌和腺癌中的表达及其临床意义。方法 利用免疫组织化学链霉菌抗生素蛋白 -过氧化物酶 (SP)法检测 3例正常人组织 ,4 8例肿瘤患者组织 ,其中包括乳腺、胃、结肠、肺腺癌、阴茎、口腔、原癌、宫颈鳞癌各 6例。结果 正常组织呈阴性染色。肿瘤组织呈现细胞膜和细胞浆阳性着色 ,E6阳性表达率在鳞癌中为 91% (2 2 / 2 4 ) ,腺癌中为 88% (16 / 18) ,直肠癌为 83% (5 / 6 ) ,在肿瘤组织中综合表达率为 89% (4 3/ 4 8) ,F9阳性表达率在鳞癌中为 91% (2 2 / 2 4 ) ,腺癌中为 83% (15 / 18) ,直肠癌为 83% (5 / 6 ) ,在肿瘤组织中综合表达率为87% (4 2 / 4 8)。结论 单抗E6,F9具有比较强的特异性 ,其高效表达可用来判定鳞癌和腺癌的生物学行为 ,并为鳞癌和腺癌的预后提供依据。

Objective To investigate the expression and clinical significance of E 6 and F 9 in squamous cell carcinoma and adenomous carcinoma. Methods Tissues of 3 normal controls and tumour tissues of 48 cases, including 6 adenomous carcinomas of the breast,stomach,colon,lung,and 6 squamous cell carcinomas of penis,oral cavity,original carcinoma and the cervices. Results Normal tissues showed negative staining. Tumour tissues showed positive staining on the cell membrane and in the cytoplasm. The positive expression ratio of E 6 in squamous cell carcinoma, 88%(16/18) in adenomous, carcinoma 83%(5/6) in retal carcinoma. The total expression rate of E 6 in tumour tissues was 89%(43/48). The positive expression rate of F 9 was 91%(22/24) in squamous cell carcinoma and 83%(15/18) in adenomous carcinoma and 83%(5/6) in rectal carcinoma. The expression of F 9 in tumour tissues was 87%(42/48). Conclusion Monoclony antibody E 6 and F 9 have high specificity. High expression rate can assist in the assessment of the biological behavior and prognosis of squamous cell carcinoma.