大豆耐盐相关基因的分离及其功能鉴定

Isolation and characterization of genes related to salt-tolerance in soybean

利用大肠杆菌功能表达体系,从大豆开花40d未成熟种子的cDNA表达文库中筛选获得了11个耐盐相关cDNA克隆.对其中3个克隆的插入片段进行测序.结果显示,其中的Gm1013为一尚未报道的新基因片段;GmRPS25 2编码的蛋白质序列与西红柿40S核糖体蛋白S25有91%的同源性;GmAIP 2编码的蛋白质序列与大豆根铝诱导蛋白SALI3 2有97%的同源性.其中GmAIP 2可表达分子量为30 8kD的多肽.pET GmAIP 2转化后的大肠杆菌在含800mmol/LNaCl和700mmol/LKCl的液体培养基中生长状况明显好于对照菌,即前者表现出较短的生长迟滞期和较高的生长量.

11 salt-tolerant cDNA colonies were isolated from a cDNA library constructed from unmatured seeds of soybean by functional screening with E.coli.Sequencing of the inserts of 3 clolonies showed that Gm1013 is an unknown new gene; GmRPS25-2 is homologous to a gene encoding a 40S ribosomal protein S25 in tomato with 91% identity and GmAIP-2 is homologous to the gene encoding an aluminium-induced protein SALI3-2 in soybean with 97% identity. GmAIP-2 could express a protein of 30.8kD. The transformant containing pET-GmAIP-2 displayed enhanced salt tolerance in liquid mediums with 800 mmol/L NaCl or 700 mmol/L KCl when compared with the control strain, and showed the shorter lag period and higher bacterial quantity.