三苯甲烷类染料降解菌的诱变选育

Mutation and breeding on the strain for degradating triphenylmethane dye

从印染废水污泥中分离筛选出对三苯甲烷类染料甲基紫具有降解活性的细菌L3,经紫外线诱变,获得高活性变异菌株UV2L3,并对其进行降解性能测定.根据对UV2L3在不同的pH值、脱色温度及时间等条件下降解甲基紫数据测定的基础上,设计正交实验,获得其降解模拟废水的最佳反应条件.研究表明,诱变菌株UV2L3在30℃及pH值为8.0的条件下,72h对浓度为50mg/L的甲基紫模拟废水的脱色率达78%以上,比出发菌株L3提高44%.脱色后模拟废水的CODcr值为110mg/L,去除率为75%.

The bacteria L_3 which has degradation activity to triphenylmethane dye methyl violet (MV) was (isolated) from textile wastewater sludge. Through mutation by ultraviolet(UV), a high active mutation (strain) UV_2L_3 was obtained. The orthogonal experimental results showed that the decolor ratio is 78% for UV_2L_3 to MV on the condition of pH 8.0,30 ℃ and 72 h, which is higher 44% than original strain L_3. CODcr of the decolorized wastewater is 110 mg/L and the decrease ratio of CODcr is about 75%.